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当前位置: 首页 > 文章 > 甘蓝型春油菜早花位点cqDTFA7a加密及其近等基因系构建 分子植物育种 2019 (21) 7047-7057
Position: Home > Articles > 甘蓝型春油菜早花位点cqDTFA7a加密及其近等基因系构建 Molecular Plant Breeding 2019 (21) 7047-7057

甘蓝型春油菜早花位点cqDTFA7a加密及其近等基因系构建

作  者：
潘云龙;柳海东
单  位：
青海大学农林科学院青海省春油菜遗传改良重点实验室国家油菜改良中心青海分中心农业农村部春油菜科学观测实验站青海省春油菜工程技术研究中心
关键词：
甘蓝型春油菜;早花位点;近等基因系
摘  要：
本研究选取NN DH系中的ZW46为母本,晚熟No.5246为父本(轮回亲本),利用cqDTFA7a位点候选区间及侧翼标记进行前景选择,其他18条染色体上的标记进行背景选择,通过连续回交及标记辅助选择方法构建甘蓝型春油菜早花位点cqDTFA7a的近等基因系.在BC2F1群体中,筛选到cqDTFA7a位点杂合的单株4株,背景回复率达到了95%以上,自交获得一个包含537单株的BC2F2群体,通过双亲基因组重测序,开发了8个InDel (insertion-deletion)标记,利用这些标记进一步加密了cqDTFA7a区间,将cqDTFA7a定位在IA7-4附近,相对遗传距离为0.1 c M,表型贡献率为47%,区间由原来的900 kb缩小到目前的300 kb.以上4个单株进一步回交获得前景位点杂合、背景回复率达到100%的单株4株,与No.5246构成了近等基因系,通过对上述4个单株自交获得一个包含843个单株的BC3F2群体,通过卡方检验,该群体花期遗传符合孟德尔遗传(3∶1),说明在该群体中,花期由一对显性基因控制.本研究为甘蓝型春油菜早花QTL位点cqDTFA7a的精细定位和候选基因挖掘奠定了基础.
作  者：
Pan Yunlong;Liu Haidong;Qinghai Spring Rape Engineering Research Center, Spring Rape Scientific Observation Experimental Station of Ministry of Agriculture and Rural Areas, The Qinghai Research Branch of the National Rapeseed Genetic Improvement Center, Key Laboratory of Qinghai Province for Spring Rapeseed Genetic Improvement, Academy of Agricultural and Forestry Sciences, Qinghai University;
关键词：
Brassica napus;;Early flowering site;;Near isogenic line
摘  要：
In this study, ZW46 in the NN DH line was selected as the female parent, and late maturity No.5246 was used as the male parent(Recurrent parent). The cqDTFA7 a locus candidate interval and flanking markers were used for foreground selection, and the markers on the other 18 chromosomes were selected for background selection. The near-isogenic line of early flowering locus cqDTFA7 a in Brassica napus L. was constructed by continuous backcrossing and marker-assisted selection. In the BC2 F1 population, 4 plants with heterozygous cqDTFA7 a locus were screened, and the background recovery rate reached over 95%. Self-acquisition to obtain a BC2 F2 population containing 537 plants, and 8 InDels(insertion-deletion) mark were developed by re-sequencing of the parental genome. Use these markers to further encrypt the cqDTFA7 a interval, positioning cqDTFA7 a near IA7-4, the relative genetic distance is 0.1 c M, the phenotypic contribution rate is 47%, and the interval is reduced from the original 900 kb to the current 300 kb. The above four individuals were further backcrossed to obtain 4 plants per plant with heterozygous foreground sites and a background recovery rate of 100%, and building a near isogenic line with No.5246. Obtaining a BC3 F2 population containing 843 individuals by self-crossing the above four individuals,through the chi-square test, the flowering inheritance of this population is consistent with Mendelian inheritance(3∶1), indicating that in this population, the flowering stage was controlled by a pair of dominant genes. This study will laid the foundation for the fine mapping and candidate gene mining of early flowering QTL locus cqDTFA7 a in Brassica napus L..

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