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Position: Home > Articles > Comparison of ELISA and LAT for Detecting Serum Antibody against Toxoplasma gondii in Pig Jilin Animal Husbandry and Veterinary Medicine 2007,28 (6) 15-16+19

ELISA和LAT检测猪血清弓形虫抗体的比较

作  者:
江涛;何会时;聂浩;陈声国;赵俊龙
单  位:
长江大学动物科学学院;华中农业大学农业微生物学国家重点实验室;荆州市畜牧局
关键词:
刚地弓形虫;rMIC3;ELISA;LAT
摘  要:
应用弓形虫重组蛋白rMIC3为抗原建立的酶联免疫吸附试验(ELISA)和乳胶凝集试验(LAT)2种方法,平行检测来自36个猪场的471份猪血清中的抗弓形虫特异性抗体。结果显示,ELISA和LAT对母猪弓形虫抗体的阳性检出率分别为42.86%(21/49)和44.90%(22/49),2种方法的阳性检出符合率为95.45%(21/22);对育肥猪的阳性检出率分别为42.06%(135/321)和43.61%(140/321),2种方法的阳性检出符合率为93.57%(131/140);对仔猪的阳性检出率分别为29.07%(30/101)和45.54%(46/101),2种方法的阳性检出符合率为63.04%(29/46)。结果表明,ELISA和LAT可用于猪弓形虫病的诊断和血清学调查,LAT更适合于仔猪弓形虫病的检测。
译  名:
Comparison of ELISA and LAT for Detecting Serum Antibody against Toxoplasma gondii in Pig
作  者:
JIANG Tao 1,2 , HE Hui-shi 3 , NIE Hao 2 , CHEN Sheng-guo 2 , ZHAO Jun-long 2 (1. College of Animal Science, Yangtze University, Jingzhou,Hubei 434025; 2. National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan,Hubei 430070; 3. Jingzhou Animal Husbandry Bureau, Jingzhou,Hubei 434100)
关键词:
Toxoplasmagondii; rMIC3; ELISA; LAT
摘  要:
The 471 serum samples of pig from 36 pig farms were tested the specific antibody against T. gondii by ELISA and LAT with the recombinant rMIC3. Seropositive rate for sows detected by ELISA and LAT were 42.86 %(21/49)and 44.90 %(22/49)respectively, the positive coincidence rate of both methods reached 95. 45 % (21/ 22), and the parameters for fattening pigs were 42.06 %(135/321), 43.61 %(140/321)and 93. 57 % (131/ 140) respectively, and for piglets were 29.07 %(30/101), 45.54 %(46/101)and 63.04 %(29/46)respectively. The results indicated that both methods of ELISA and LAT could be applied to serodiagnosis and seroprevalence for porcine Toxoplasmosis, and LAT may fit for serodiag- nosis of piglet Toxoplasmosis.

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