作 者：
王欢;李杨;江连洲;王妍;王中江;冯红霞;冯丹;刘海英
关键词：
火麻籽;水酶法;油得率;响应面分析;脂肪酸含量
摘 要：
以去皮火麻籽为原料,采用多种酶(Protex 6L、Protex 7L、Alcalase 2.4L、Viscozyme L、八宝威2X)对火麻仁原料进行水解,提取游离油,油得率分别是3.62%、36.88%、17.05%、50.98%、33.33%。以分别得到的乳状液、水解液、沉淀中油含量作为分析依据,选出Viscozyme L酶为最优使用酶。以游离油得率为指标、用响应面法进行优化,确定最优水酶法提取火麻仁游离油工艺条件为加酶量7 000 U/g、酶解温度60℃、pH 5、料液比1∶6、酶解时间3 h。通过气相色谱法共分析出11种脂肪酸,其中饱和脂肪酸以棕榈酸、花生酸、硬脂酸为主,不饱和脂肪酸以油酸、亚油酸、α-亚麻酸为主,其中亚油酸含量最多,相对含量约为47.5%。
译 名：
Optimization of aqueous enzymatic extraction of hemp seed oil and analysis of its fatty acid composition
作 者：
Wang Huan;Li Yang;Jiang Lianzhou;Wang Yan;Wang Zhongjiang;Feng Hongxia;Feng Dan;Liu Haiying;Food Science College of Northeast Agricultural University;The National Research Center of Soybean Engineering and Technology;
关键词：
hemp seed;;aqueous enzymatic method;;oil yield;;response surface methodology;;fatty acid content
摘 要：
The yields of hemp seed oil from peeled hemp seeds by aqueous enzymatic extraction with Protex 6 L,Protex 7 L,Alcalase 2.4 L,Viscozyme L and Babaowei 2 X were 3.62%,36.88%,17.05%,50.98%,and33.33%,respectively.Viscozyme L was chosen as the best enzyme according to the oil contents in the emulsions,hydrolysates and precipitates.The enzymatic hydrolysis process was optimized by response surface methodology to obtain maximum free oil yield.As a result,the optimal hydrolysis conditions were determined as 7 000 U/g of enzyme dosage,60 ℃,initial p H 5,1∶ 6 of solid/liquid ratio and 3 h.By using GC analysis,11 fatty acids were detected in the seed oil,including saturated fatty acids(mainly palmitic,arachidic and stearic acid) and unsaturated fatty acids(mainly oleic,linoleic and α-linolenic acid).Among these fatty acids,the most abundant was linoleic acid with a relative content of 47.5%.
