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当前位置: 首页 > 文章 > 牛膝多糖对断奶仔猪抗菌肽Protegrin-1 mRNA表达的影响 动物营养学报 2008,20 (1) 84-88
Position: Home > Articles > The Effect of Achyranthes Bidentata Polysaccharides on Protegrin-1mRNA Expression in Weaned Piglets Chinese Journal of Animal Nutrition 2008,20 (1) 84-88

牛膝多糖对断奶仔猪抗菌肽Protegrin-1 mRNA表达的影响

作  者：
赵玉蓉;陈清华;贺建华;范志勇;周灿;刘祝英
单  位：
湖南农业大学动物科技学院
关键词：
半定量RT-PCR;牛膝多糖;断奶仔猪;抗菌肽;Protegrin-1;基因表达
摘  要：
本文旨在探讨牛膝多糖(ABPS)对断奶仔猪抗菌肽Protegrin-1(PG-1)mRNA表达的影响。试验采用单因子设计,按照日粮处理因素不同,设对照组、抗生素组、0.05%、0.10%和0.15%牛膝多糖组,共5个处理。选用28日龄杜长大仔猪160头,随机分入5个处理,每个处理4个重复,每个重复8头猪(公母各半)。采用半定量RT-PCR法,以18SrRNA为内标,检测PG-1mRNA相对表达量。结果表明:日粮中添加牛膝多糖均不同程度地促进断奶仔猪骨髓抗菌肽PG-1mRNA的表达。与对照组相比,0.05%、0.10%和0.15%牛膝多糖组分别使断奶仔猪PG-1mRNA的相对表达量增加14.9%(P>0.05)、36.4%(P<0.05)和7.3%(P<0.05);与抗生素组相比,其PG-1mRNA的相对表达量分别增加7.8%(P>0.05)、27.9%(P>0.05)和19.4%(P>0.05)。即牛膝多糖能显著促进断奶仔猪骨髓抗菌肽PG-1mRNA表达,且日粮中以0.10%添加量为适宜。
译  名：
The Effect of Achyranthes Bidentata Polysaccharides on Protegrin-1mRNA Expression in Weaned Piglets
作  者：
ZHAO Yu-rong CHEN Qing-hua HE Jian-hua FAN Zhi-yong ZHOU Can LIU Zhu-ying(College of Animal Science and Technology,Hunan Agriculture University,Changsha 410128,China)
关键词：
Semi-quantitative RT-PCR;Achyranthes bidentata polysaccharides;Weaned piglets;Antibacterial peptide;Protegrin-1;Gene expression
摘  要：
To investigate the effect of Achyranthes bidentata polysaccharides(ABPS)on expression of antibacterial peptide Protegrin-1(PG-1)mRNA in weaned piglets,according to the different dietary factors,five test groups were used,including control group,antibiotics group,0.05%,0.10% and 0.15% ABPS groups.One hundred and sixty weaned piglets(Duroc×(Landrace×Yorkshine))with age of 28 days were randomly allotted to five test groups with four replicates in each group and eight weaned piglets in each repeat.An optimized semi-quantitative RT-PCR was constructed to detect the relative expression levels of PG-1 mRNA by using 18S rRNA as inner control.The results showed that PG-1 mRNA expression could be enhanced to a different extent with ABPS in weaned piglets.Compared with control group,0.05%,0.10% and 0.15% ABPS enhanced the PG-1 mRNA expression by 14.9%(P>0.05),36.4%(P<0.05)and 27.3%(P<0.05)respectively;Compared with antibiotics group,0.05%,0.10% and 0.15% ABPS increased the PG-1 mRNA expression by 7.8%(P>0.05),27.9%(P>0.05)and 19.4%(P>0.05)respectively.Above results suggested that,ABPS remarkably increased relative expression of PG-1 mRNA in femur marrow of weaned piglets,and the appropriate ABPS addition was 0.10%.

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