关键词：
侧柏籽油;超声波辅助提取;工艺条件;响应面分析;气相色谱-质谱分析
摘 要：
以环己烷为提取溶剂,采用响应面法(RSM)优化超声波辅助提取侧柏籽油的工艺条件,在单因素试验基础上,选取液料比、提取时间、提取温度、超声波功率为影响因素,以侧柏籽油提取率为响应值,应用中心组合试验设计(central composite design,CCD)建立数学模型,进行响应面分析,并采用GC-MS测定侧柏籽油的脂肪酸组成。结果表明,提取侧柏籽油的优化工艺条件为:液料比7:1(mL/g)、提取时间38min、提取温度55℃、超声波功率270W,在此工艺条件下,侧柏籽油提取率为93.47%;GC-MS测定结果表明侧柏籽油中富含不饱和脂肪酸,总含量达到84.37%,其中油酸、亚油酸和亚麻酸的含量分别为28.41%、11.40%和44.56%。
译 名：
Ultrasound-assisted Extraction and Fatty Acid Composition of Biota Orientalis Seed Oil
作 者：
MA Cheng-jin1,2,WU Zhu-qing2,HUANG Wei2,XIAO Zhuo-bing1,HUANG Qun2 (1. Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Zhangjiajie 427000, China; 2.Institute of Food Science, Jishou University, Jishou 416000, China)
关键词：
Biota orientalis seed oil;ultrasound-assisted extraction;technical conditions;response surface analysis;GC-MS
摘 要：
Oil in Biota orientalis seed was extracted using n-hexane with the assistance of ultrasound. Effects of liquid-to-material ratio, extraction time, extraction temperature and ultrasound power on the yield of oil were investigated by conducting single factor tests. Subsequently, a central composite design was employed and the results were analyzed by using response surface methodology. The optimal extraction conditions were as follows: liquid-to-material ratio 7:1 , extraction time 38 min, extraction temperature 55 ℃,ultrasound power 270 W. Under such conditions, the yield of the oil was 93.47%. Gas chromatography-mass spectrometry analysis indicated that the oil was abundant in unsaturated fatty acids, accounting for 84.37% of the total fatty acids and consisting of 28.4.1% of oleic acid , 11.40% of linoleic acid and 44.56% of linolenic acid.
