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Position: Home > Articles > CLUSTER TYPING OF THE LEUCOCYTE ANTIGENS IN DAIRY CATTLE Scientia Agricultura Sinica 1989,22 (4) 84-87

奶牛白细胞抗原的聚类分型

作  者:
唐鼎恩;秦志锐;齐广海;杨启云;董志兵
单  位:
中国农业科学院畜牧研究所
关键词:
白细胞抗原;聚类分型;主要组织相容性;微量淋巴细胞毒试验
摘  要:
哺乳动物的白细胞抗体可从怀孕母畜的血清中获得。这是因为母体受到本身不具有的父源性白细胞抗原通过胎儿刺激母体使之产生抗体。本试验取自天津两个牛场502头不同胎次的怀孕母牛血清。采用国际标准微量淋巴细胞毒试验方法。用23头北京地区随机牛的淋巴细胞初步筛选,获得阳性血消223份,总阳性率为44.4%(223/502)。将初筛选出的阳性血清再与50头北京地区随机牛的淋巴细胞进行微量淋巴细胞毒试验,将反应结果按白细胞分型聚类程序进行电子计算机聚类。得到了14组不同特异性的血清。这14组血清我们暂命名为T1-T14。发现黑白花奶牛的少见抗原为T3和T4型;常见抗原为T10型。这14组血清做为分型用标准血清,可用于品种鉴定 、亲子关系鉴定、选育优良和抗病品种及疾病诊断等方面。由于品种较单一以及配种用的公牛头数有限还有未检测出的抗原型,今后应继续扩大筛选范围,使之分型标准血清更丰富,应用范围更厂泛。
译  名:
CLUSTER TYPING OF THE LEUCOCYTE ANTIGENS IN DAIRY CATTLE
作  者:
Tang Dingen Qin Zhirui Qi Guanghai Yang Qiyun (Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing)
关键词:
Leucocyte antigens; Cluster analysis; ajor histocompatibility; Microlymphocytotoxic test
摘  要:
The 502 pregnant dairy cattle sera from 2 farms in Tianjin were tested by the international microlymphocytotoxic method to detect parentally derived leucocyte antigens. After reacting with 23 random cattle lymphocytes, it was found that the total positive percentage was 44.4%. The positive sera were then reacted with50random cattle lymphocytes by the international microlymphocytotoxic method. The14 specific serotypes (TI-T14) were found after cluster analysis. Among them, T10 is the most common antigen, and T3 & T4 are the rare ones. They can be used as standard sera in the identification of breeds and parentage, diagnosis of disease, and breeding of disease resist ance. The range of antigen screening remains to be widened to increase types of standard sera.

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