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Position: Home > Articles > RAPD Analysis of the Genomic of Parent Ducks and Their Hybrids Journal of Henan Agricultural Sciences 2006,35 (1) 104-107

RAPD技术分析亲本鸭及其杂交后代的基因组变化

作  者:
苏瑛;刘楚吾;叶昌辉;刘丽;张锦;蔡笛扬
单  位:
广东省湛江市坡头区畜牧局;广东海洋大学
关键词:
樱桃谷鸭;麻鸭;杂交;RAPD;基因组
摘  要:
对樱桃谷鸭和麻鸭及其杂交子代(樱♂×麻♀)3个样本的基因组DNA进行了RAPD标记分析,探讨了杂种优势产生的分子遗传机制。从120个随机引物中筛选出20个引物进行扩增,460条扩增带中,有330条带具有多态性。群体内相似性指数分别为0.7888(樱桃谷鸭)、0.7629(麻鸭)和0.8227(F1)。F1与母本的相对遗传距离为0.3300,与父本为0.3505,表明F1偏向母本。
译  名:
RAPD Analysis of the Genomic of Parent Ducks and Their Hybrids
作  者:
SU Ying~1,LIU Chu-wu~1,YE Chang-hui~1,LIU Li~1,ZHANG Jin~2,CAI Di-yang~2 (1.Guangdong Ocean University,Zhanjiang 524088,China;2.Zhanjiang Potou Bureau of Animal Husbandry,Zhanjiang 524045,China)
关键词:
Cherry Valley Meat duck;Partridge ducks;Hybrid;RAPD;Genome
摘  要:
The genomic changes of parent ducks and their hybrid were analyzed with RAPD method.20 from 120 random primers were used to amplify genomic DNA of Cherry Valley Meat duck,Partridge ducks and their hybrids(C♂ × P♀).460bands were produced and 330 among them were polymorphism(71.73%).The inter-population similarity indexes were 0.7888(C),0.7629(P)and(0.8227)(F_1))respectively,which showed greater variation among the parent and their hybrids.And the genetic distance between F_1 and its mother was 0.3300,while it was 0.3505 with its father,which indicated that the genome was more similar to its mother.

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