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Position: Home > Articles > Study on technique of decoloring of polysaccharide from Acanthopanax obovatus Hoo Science and Technology of Food Industry 2013 (4) 296-299

倒卵叶五加多糖脱色工艺研究

作  者:
李小蓉;黄毓娟;权彦
单  位:
陕西中医学院药学院
关键词:
倒卵叶五加;多糖;脱色
摘  要:
采用水提醇沉法提取倒卵叶五加多糖,分别采用双氧水法和大孔树脂法对倒卵叶五加多糖进行了脱色研究。在单因素实验的基础上进行了正交实验,得到双氧水法脱色的工艺条件为:温度60℃,时间3h,pH=10.0,样液比1∶1。在该条件下多糖脱色率为84.45%,多糖保留率为90.94%。树脂法脱色的工艺条件为LS-700B树脂,温度45℃,溶液pH=7.0。在该条件下的脱色率达到79.50%,多糖保留率为82.80%,蛋白脱除率达到79.10%。实验结果表明,双氧水法是一种相对较优的脱色方法。
译  名:
Study on technique of decoloring of polysaccharide from Acanthopanax obovatus Hoo
作  者:
LI Xiao-rong,HUANG Yu-juan,QUAN Yan(College of Pharmacy,Shaan Xi University of Chinese Medicine,Xianyang 712046,China)
关键词:
Acanthopanax obovatus Hoo;polysaccharide;decoloration
摘  要:
Polysaccharide of Acanthopanax obovatus Hoo was extracted with water and ethanol.Decoloration of Acanthopanax obovatus Hoo was studied with hydrogen peroxide solution method and the method of resin.With the single-factor and orthogonal test,the optimal technological conditions for Hydrogen peroxide solution method were determined as follows:temperature 60℃,time 3h,pH =10.0,sample liquid ratio 1:1.Under these conditions,the results were as follows:84.45% decoloration rate,90.94% polysaccharides holding rate.While the optimal technological conditions for the method of resin were determined as follows:LS-700B,temperature 45℃,pH=7.0,experimental results were as follows:decoloration rate:79.50%,polysaccharides holding rate:82.80%,protein removal rate:79.10%.The results indicated hydrogen peroxide solution method was a relatively optimized decoloring method.

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