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Position: Home > Articles > A Preliminary Study on Rapid Diagnosis of Spring Viremia of Carp Virus by Indirect ELISA Journal of Jinling Institute of Technology 2012,28 (4) 85-87

间接ELISA方法快速检测鲤春病毒(SVCV)的初步研究

作  者:
郭闯;陈静;刘训猛;邹勇
单  位:
江苏省水产技术推广站
关键词:
超速离心;鲤春病毒;间接ELISA;细胞培养
摘  要:
以超速离心纯化的鲤春病毒(SVCV)免疫大白兔,制备了兔抗SVCV免疫血清,在此基础上建立了快速检测SVCV的间接ELISA方法,对40份经过细胞培养和RT-PCR方法检毕的SVC样品(8份阳性,32份阴性)进行验证,符合率100%。
译  名:
A Preliminary Study on Rapid Diagnosis of Spring Viremia of Carp Virus by Indirect ELISA
作  者:
GUO Chuang,CHEN Jing,LIU Xun-meng,ZOU Yong(Jiangsu Aquatic Technical Promotion Station,Nanjing 210036,China)
关键词:
ultracentrifugation;Spring Viremia of Carp Virus;indirect-ELISA;cell culture
摘  要:
With Spring Viremia of Carp Viruses(SVCV) purified by ultracentrifugation,an experimental rabbit is immunized to produce anti SVCV serum.On that basis,a method to rapidly detect SVCV by indirect-ELISA is established,and forty samples(eight SVCV positive and thirty-two negative) are verified with 100% accordance rate,which are first detected by cell culture and RT-PCR.

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