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当前位置: 首页 > 文章 > 不同磷效率柱花草基因型对外源DNA活化利用能力的比较分析 分子植物育种 2018 (4) 1085-1091
Position: Home > Articles > Comparative Analysis of Exogenous DNA Utilization in Stylosanthes Genotypes with Contrasting P Efficiency Molecular Plant Breeding 2018 (4) 1085-1091

不同磷效率柱花草基因型对外源DNA活化利用能力的比较分析

作  者：
刘攀道;董荣书;丁西朋;唐军;郇恒福;白昌军;陈志坚;刘国道
单  位：
中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点实验室
关键词：
柱花草;磷效率;低磷胁迫;有机磷;紫色酸性磷酸酶
摘  要：
本研究以不同磷效率的4个柱花草基因型L6、L8、L10和Y3为材料,分析不同磷源处理对柱花草生物量、全磷含量、根系酸性磷酸酶活性和紫色酸性磷酸酶基因Sg PAPs表达的影响。结果表明,相对于不加磷处理(-P),添加外源DNA(+DNA)和速效磷处理(+P)均显著增加了柱花草的干重和全磷含量,但是,在添加外源DNA处理下,磷高效基因型L10与Y3的干重和全磷含量显著高于磷低效基因型L6与L8。在不加磷和添加外源DNA处理下,柱花草的根系内源和分泌酸性磷酸酶活性均显著增加,但L10和Y3的根系分泌酸性磷酸酶活性显著高于L6和L8。Sg PAP7和Sg PAP10在4个柱花草基因型的根系中均受低磷胁迫(不加磷和添加外源DNA处理)诱导上调表达,且在L10和Y3中的相对表达量显著高于L6和L8。以上结果表明,磷高效柱花草基因型L10和Y3具有较高的根系分泌酸性磷酸酶活性和较强的活化利用外源DNA能力。当体内磷缺乏时,柱花草通过增加Sg PAP7和Sg PAP10的表达来调控酸性磷酸酶活性,从而增加对外源DNA的活化利用。本研究为磷高效柱花草的选育和遗传改良提供了理论依据和候选基因资源。
译  名：
Comparative Analysis of Exogenous DNA Utilization in Stylosanthes Genotypes with Contrasting P Efficiency
作  者：
Liu Pandao;Dong Rongshu;Ding Xipeng;Tang Jun;Huan Hengfu;Bai Changjun;Chen Zhijian;Liu Guodao;Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences & Key Laboratory of Crop Gene Resources andGermplasm Enhancement in Southern China, Ministry of Agriculture;
关键词：
Stylosanthes;;P efficiency;;Low P stress;;Organic P;;Purple acid phosphatase
摘  要：
Plant biomass, phosphorus(P) content, root acid phosphatase(APase) activity and transcripts of SgPAPs were analyzed in four stylo(Stylosanthes) genotypes, L6, L8, L10 and Y3, with contrasting P efficiency, under different P treatments. Results showed that plant dry weight and total P content significantly increased with application of both exogenous DNA(+DNA) and available P(+P) compared to the treatment of no P(-P) addition. Plant dry weight and total P content in L10 and Y3 were higher than those in L6 and L8 with exogenous DNA treatment. Root internal and root secreted APase activities significantly increased in the treatment of exogenous DNA and no P treatments. Additionally, root secreted APase activities in L10 and Y3 were higher than those in L6 and L8, and expression levels of SgPAP7 and SgPAP10 were up-regulated by P deficiency in the four tested stylo roots.Furthermore, there was a higher abundance of transcripts of SgPAP7 and SgPAP10 in L10 and Y3 than those in L6 and L8 under P deficiency. These resul ts suggested that the P efficient stylo genotypes, L10 and Y3, exhibitedsuperior root secreted APase activities and DNA utilization capability, which might be regulated by the expressions of SgPAP7 and SgPAP10 under low P stress. Our results provided theoretical basis and gene resources for selecting and improving stylo varieties adapted to low P stress.

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