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Position: Home > Articles > Screening and Breeding of An Edible High-yield Protein-producing Strain of Candida utilis and Optimization of Its Fermentation Conditions FOOD SCIENCE 2009,30 (21) 210-213

高产食用蛋白质产朊假丝酵母菌的选育及发酵条件优化

作  者:
何东东;张坤生
单  位:
天津市食品生物技术重点实验室天津商业大学食品科学与工程系
关键词:
紫外诱变;发酵;优化
摘  要:
通过对产朊假丝酵母菌的紫外诱变,筛选蛋白质含量较高的可食用菌株。结果表明:在15W紫外线(30cm)处,照射120s,致死率为89%,得到蛋白质含量为33%的诱变菌株,比诱变前的22.9%提高了37.12%。对此菌种进行稳定性测试(传代10次并进行蛋白质测定),性质稳定;对诱变后的发酵条件:碳源、氮源、培养温度和溶氧量(培养箱转速)进行优化,最终得到采用葡萄糖为碳源、硫酸铵和酵母浸膏粉的混合物为氮源、培养温度为32℃、转速为160r/min时培养出的蛋白质含量及菌体生物量都在较高水平,蛋白质含量为37%。
译  名:
Screening and Breeding of An Edible High-yield Protein-producing Strain of Candida utilis and Optimization of Its Fermentation Conditions
作  者:
HE Dong-dong,ZHANG Kun-sheng (Tianjin Key Laboratory of Food Biotechnology, Department of Food Science and Engineering, Tianjin University of Commerce, Tianjin 300134, China)
关键词:
UV mutagenesis;fermentation;optimization
摘  要:
This study aimed to screen and breed an edible strain of Candida utilis exhibiting high protein content via UV mutagenesis. Results showed that the exposure to 15 W UV light (at a distance of 30 cm) for 120 s led to a lethal rate of up to 89% and a new mutant strain B2 was obtained with a high protein content of 33%, which was higher than before mutagenesis (22.9%) by 37.12%. Analysis on protein content showed high genetic stability of this mutant strain after 10 passagings. The optimal B2 fermentation, which resulted in high protein content in mycelia (37%) and biomass of Candida utilis was achieved through culture at 32 ℃ with a shaking speed of 160 r/min using glucose as carbon source and (NH4)2SO4 and yeast extract powder as nitrogen sources.

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