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当前位置: 首页 > 文章 > 红酵母发酵产类胡萝卜素条件的优化 食品科学 2009,30 (15) 176-179
Position: Home > Articles > Orthogonal Array Optimization of Carotenoid Production by Rhodotorula sp. FOOD SCIENCE 2009,30 (15) 176-179

红酵母发酵产类胡萝卜素条件的优化

作  者：
陈欣
单  位：
江苏科技大学生物与环境工程学院
关键词：
红酵母;类胡萝卜素;紫外诱变;优化
摘  要：
为提高红酵母发酵产类胡萝卜素的产量,对其进行紫外诱变处理并进行发酵条件的优化实验。结果显示:最佳培养条件为葡萄糖浓度2.5%、培养温度28℃、培养时间120h、起始pH5.0,在此条件下,类胡萝卜素产量达到11.61mg/L。结论:诱变菌株在生物量、类胡萝卜素含量和产量上较出发菌株均有所提高。
译  名：
Orthogonal Array Optimization of Carotenoid Production by Rhodotorula sp.
作  者：
CHEN Xin (School of Biology and Environmental Engineering,Jiangsu University of Science and Technology,Zhenjiang 212003,China)
关键词：
Rhodotorula sp.;carotenoid;UV mutagenization;optimization
摘  要：
A strain of Rhodotorula sp. was mutagenized with UV radiation and the mutant stain was adopted to produce carotenoid. The effects of glucose concentration and initial pH of fermentation medium,temperature and fermentation time on carotenoid production were investigated by single-factor method. An orthogonal array design leading to a set of 9 combinations of the four variables was conducted to attain the highest carotenoid yield. The UV mutagenization for 30 s resulting in about 80% mutation rate was chosen to obtain high-yield carotenoid-producing mutant strains. The optimal conditions for improved carotenoid production was determined as follows:glucose concentration 2.5%,initial pH 5.0,temperature 28 ℃,and fermentation time 120 h. Under these conditions,the carotenoid yield was found to reach 11.61 mg/L,higher than that by starting stain.

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