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当前位置: 首页 > 文章 > 贵州矮马鼠伤寒沙门氏菌的分离鉴定及其致病力研究 中国畜牧兽医 2016,43 (10) 2756-2761
Position: Home > Articles > Isolation,Identification and Pathogenicity of Salmonella typhimurium from Guizhou Pony China Animal Husbandry & Veterinary Medicine 2016,43 (10) 2756-2761

贵州矮马鼠伤寒沙门氏菌的分离鉴定及其致病力研究

作  者：
邓显佑;薛艳;王嘉福;冉雪琴
单  位：
贵州大学农业生物工程研究院;贵州大学动物科学学院
关键词：
贵州矮马;鼠伤寒沙门氏菌;侵袭蛋白A基因;致病力
摘  要：
为了探究贵州矮马的腹泻病原,试验以腹泻粪样为材料进行病原菌的分离鉴定。经SS和XLD培养基筛选,得到1株菌株S6,经革兰氏染色、生理生化检验以及16SrRNA基因序列比对分析,S6菌株可在SS和XLD琼脂培养基表面生长,革兰氏染色呈阴性;生理生化检验结果与沙门氏菌的生化特性相符;16SrRNA基因序列与已知的鼠伤寒沙门氏菌ATCC 13311(登录号:NR_119108)的亲缘关系最近,序列同源性为99%。形态学和分子系统学分析表明,该菌株为鼠伤寒沙门氏菌。采用PCR方法从S6菌株基因组中得到侵袭蛋白A(invasion protein A,invA)基因,与已知基因序列有6~8bp不一致,编码的氨基酸发生了一个替换。通过改良寇氏法测定S6菌株对小鼠的半数致死量(LD50)为4.71×102 CFU,属于强致病力菌株。推测S6 invA基因的变异可能与菌株的毒力强弱有关。贵州矮马群体中沙门氏菌的检出率为57%。本研究提示贵州矮马的腹泻病原之一可能是强毒力的鼠伤寒沙门氏菌。
译  名：
Isolation,Identification and Pathogenicity of Salmonella typhimurium from Guizhou Pony
作  者：
DENG Xian-you;XUE Yan;WANG Jia-fu;RAN Xue-qin;Institute of Agricultural Bioengineering,Guizhou University;Tongren College;College of Animal Science,Guizhou University;
关键词：
Guizhou pony;;Salmonella;;invasive protein Agene;;pathogenicity
摘  要：
To investigate the reason of the diarrhea in Guizhou pony,we used the feces of pony as experimental material to isolate and detect pathogenic bacteria.S6 strain was isolated from SS and XLD medium,and identified using Gram staining,biochemical tests and molecular phylogeny methods.The results showed that S6 strain could growth on SS and XLD medium,and the Gram staining was negative.Biochemical test suggested that its phenotype features were accordance with Salmonella.The 16 SrRNA gene sequence of S6 strain was determined in a nucleotide sequence identity of 99% with Salmonella typhimurium ATCC 13311(NR_119108).Based on the morphological and molecular phylogenetic results,the strain was identified as Salmonella typhimurium S6 strain.It was virulent to mice with the median lethal dose(LD50)of 4.71×102 CFU.Then,we amplified the invasion protein A(invA)gene by PCR method.The invA gene isolated from S6 strain contained 6to 8bp different from the known gene,which resulted in only one amino acid substitution.The mutant sites of invA gene might attribute to the pathogenicity of S6 strain.The detection rate of Salmonella was 57%in Guizhou pony population.It was inferred that the diarrhea in Guizhou pony might be caused by virulent Salmonella typhimurium.

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