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当前位置: 首页 > 文章 > 贵州矮马与伊犁马生长激素基因5’侧翼区的结构特征 贵州农业科学 2012,40 (4) 151-155
Position: Home > Articles > Structure Characteristics of 5'Flanking Region of Growth Hormone Gene of Guizhou Pony and Ili Horse Guizhou Agricultural Sciences 2012,40 (4) 151-155

贵州矮马与伊犁马生长激素基因5’侧翼区的结构特征

作  者：
王三铭;王嘉福;田松军;王荣明;党珍;冉雪琴
单  位：
贵州省紫云县农业局;贵州大学动物科学学院
关键词：
生长激素基因;5’侧翼区;结构特征;贵州矮马;伊犁马
摘  要：
为了摸索贵州矮马生长发育的调控机制,探明贵州矮马与伊犁马生长激素基因5’侧翼区的结构特征,以4～5龄的贵州矮马和7～8龄的新疆伊犁马为研究对象,采用直接测序法从两种马基因组中克隆了生长激素(GH)基因5’侧翼区的DNA序列,并进行生物信息学分析。结果表明:1)从基因组中克隆的711bp片段为GH基因5’侧翼区序列;2)两个马品种的GH基因5’侧翼区中都存在TATA box、CAATbox、GC box和Octamer位点等真核生物启动子结构;3)从伊犁马GH基因5’侧翼区中检测到1个位于30bp处的突变位点发生了G→C颠换,由此新增了1个转录因子upstream stimulatory factor(USF)结合位点;4)从贵州矮马和伊犁马GH基因的5’侧翼区序列中发现了MZF1、CdxA、Nkx-2、AML-1a和c-Myc等19种共58个转录因子的结合位点。结论:克隆的711bp基因序列中存在大量的转录调控元件。
译  名：
Structure Characteristics of 5'Flanking Region of Growth Hormone Gene of Guizhou Pony and Ili Horse
作  者：
WANG San-ming1,WANG Jia-fu1,2,TIAN Song-jun3, WANG Rong-ming3,DANG Zhen1,RAN Xue-qin1(1.College of Animal Science,Guizhou University,Guiyang,Guizhou 550025;2.Guizhou Key Laboratories for Agricultural Bioengineering,Guiyang,Guizhou 550025;3.Ziyun Agricultural Bureau,Ziyun,Guizhou 561600,China)
关键词：
growth hormone gene;5' flanking region;structure characteristics;Guizhou pony;Ili horse
摘  要：
The DNA sequence of 5' flanking region of growth hormone gene from Guizhou ponies with 4~5 years old and Ili horses with 7~8 years old was cloned and analyzed to study the structure characteristics of 5' flanking region of growth hormone gene from different horse types and probe the regulation mechanism for growth and development of Guizhou pony.The results showed that: 1)The sequence of 5' flanking region of growth hormone gene with 711 bp was successfully cloned from the genome;2)There were TATA box,CCAAT box,GC box and Octamer euckaryote promoters in 5' flanking region of growth hormone gene of Guizhou pony and Ili horse;3) G→C transversion occurred at 30 bp mutation site of 5' flanking region of growth hormone gene from Ili horse,which resulted in a new transcription factor upstream stimulatory factor(USF)binding site;4) 58 transcription factor binding sites including MZF1,CdxA,Nkx-2,AML-1a,c-Myc and so on were discovered in 5' flanking region of growth hormone gene from Guizhou pony and Ili horse.In conclusion: There are many transcription regulatory elements in the cloned 711 bp gene sequence from Guizhou pony and Ili horse.

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