当前位置: 首页 > 文章 > 牛磺鹅去氧胆酸对佐剂性关节炎大鼠成纤维样滑膜细胞脂皮素-1基因表达的影响 中国畜牧兽医 2012,39 (4) 17-21
Position: Home > Articles > Effect of TCDCA on Lipocortin-1 Expression in Fibroblast-like Synoviocytes in Rats with Adjuvant Arthritis China Animal Husbandry & Veterinary Medicine 2012,39 (4) 17-21

牛磺鹅去氧胆酸对佐剂性关节炎大鼠成纤维样滑膜细胞脂皮素-1基因表达的影响

作  者:
关红;毛伟;李培锋;刘明强
单  位:
内蒙古农业大学兽医学院
关键词:
牛磺鹅去氧胆酸;脂皮素-1;荧光定量RT-PCR;成纤维样滑膜细胞
摘  要:
观察牛磺鹅去氧胆酸(taurochenodeoxycholic acid,TCDCA)对大鼠佐剂性关节炎(adjuvant arthritis,AA)模型成纤维样滑膜(fibroblast-like synoviocytes,FLS)细胞脂皮素-1(lipocortin 1,LC-1)基因表达的影响,并探讨TCDCA对AA大鼠FLS的作用机制。制备AA大鼠模型,采用组织块培养法分离培养AA大鼠FLS,应用荧光定量RT-PCR技术检测TCDCA对AA大鼠FLS细胞LC-1mRNA表达的影响。与模型组相比,TCDCA作用组AA大鼠FLS细胞LC-1mRNA的表达显著高于模型组(P<0.05)。说明TCDCA能显著促进AA大鼠FLS细胞LC-1mRNA的表达。
译  名:
Effect of TCDCA on Lipocortin-1 Expression in Fibroblast-like Synoviocytes in Rats with Adjuvant Arthritis
作  者:
GUAN Hong1,2,MAO Wei1,2,LI Pei-feng1,2,LIU Ming-qiang1,2(1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;2.Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,Ministry of Agriculture,Hohhot 010018,China)
关键词:
taurochenodeoxycholic acid;lipocortin-1;fluorescent quantitative RT-PCR;fibroblast-like synoviocytes
摘  要:
To observe the effect of taurochenodeoxycholic acid(TCDCA) on lipocortin 1(LC-1) expression in fibroblast-like synoviocytes(FLS) cell in rats with adjuvant arthritis(AA),and to explore its effect mechanism.Adjuvant arthritis was induced by Freund's complete adjuvant in rats.The synovial cells from AA rats were cultured with synovial tissue.The effect of TCDCA on the expression of LC-1 mRNA was examined quantitatively by fluorescent quantitative RT-PCR.TCDCA decreased significantly the expression of LC-1 mRNA in AA FLS cell(P<0.05),compared with that in model group.TCDCA can significantly promote the expression of LC-1 mRNA in AA FLS cell.

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