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Position: Home > Articles > Constructing the cDNA Library of Dolly Varden Charr(Salvelinus malma) Hebei Fisheries 2007 (12) 15-17

花羔红点鲑cDNA文库的构建

作  者:
郑伟;杜晓燕;李秀颖
单  位:
吉林省水产科学研究院
关键词:
花羔红点鲑;cDNA;文库
摘  要:
以花羔红点鲑为研究对象,从肝、肾和肌肉中提取总RNA,进一步纯化出mRNA,反转录成cD-NA,构建其cDNA文库,为进行遗传基因的研究奠定基础,同时挽救和保存濒危冷水性鱼类资源花羔红点鲑的遗传信息。
译  名:
Constructing the cDNA Library of Dolly Varden Charr(Salvelinus malma)
作  者:
Zheng Wei,Du Xiaoyan,Li Xiuying (Ji Lin Provincal Fisheries science institute,Changchun 130033,China)
关键词:
Dolly Varden Charr(Salvelinus malma) cDNA Library
摘  要:
Dolly Varden Charr(Salvelinus malma) were used as experimental objects in this study.Their total RNA were extracted from liver,kidney and muscle.Purified the mRNA and reverse transcripted to cDNA.Constructing the cDNA library to found the study of genetic gene.Meanwhile retrieve and conservate the hereditary information of Salvelinus malma,Which is the endangered cool-water fish.

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