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当前位置: 首页 > 文章 > 苏云金芽孢杆菌A322菌株发酵培养基和发酵条件的优化 热带生物学报 2014,5 (3) 253-259
Position: Home > Articles > Optimization of Bacillus thuringiensis A322 Strain Fermentation Medium and Cultural Conditions Journal of Tropical Biology 2014,5 (3) 253-259

苏云金芽孢杆菌A322菌株发酵培养基和发酵条件的优化

作  者：
张路路;朱朝华;郭刚
单  位：
中国热带农业科学院海口实验站;海南大学
关键词：
苏云金芽孢杆菌;Bacillus thuringiensis;培养基;发酵条件
摘  要：
以菌体生长对数末期发酵液OD600值为优化标准,通过单因素和正交试验方法对前期室内实验筛选得到的对棉铃虫(Helicoverpa armigera Hubner)有较高活性的苏云金芽孢杆菌Bt A322菌株进行培养基优化,并进行发酵。结果表明,最佳培养基配方为0.5%黄豆粉+0.5%酵母粉+0.5%葡萄糖+0.5%胰蛋白胨+0.2%碳酸钙+0.1%磷酸二氢钾+0.1%硫酸镁。最佳培养条件为培养时间20 h,初始p H值7.5,发酵温度30℃,摇床转速为200 r·min-1,接种量3%,250 m L三角瓶适宜装35 m L培养基。优化后的菌体生长量OD600值比优化前的提高了44.7%。
译  名：
Optimization of Bacillus thuringiensis A322 Strain Fermentation Medium and Cultural Conditions
作  者：
ZHANG Lulu;ZHU Chaohua;GUO Gang;Institute of Plant and Environmental Protection,Hainan University;Haikou Experiment Station,Chinese Academy of Tropical Agricultural Sciences;
关键词：
Bacillus thuringiensis;;medium;;fermentation condition;;optimization
摘  要：
Bacillus thuringiensis Berliner strain A322 with higher activity against Helicoverpa armigera Hubner screened indoor was cultured on media through single-factor and orthogonal experiment to optimize cultural medium based on the optimization standard of OD600 of the fermented liquid of B. thuringiensis at the late logarithmic phase. The optimum medium is 0. 5% soybean meal,0. 5% yeast extract,0. 5% glucose,0. 5% tryptone,0. 2% calcium carbonate,0. 1% potassium dihydrogen phosphate,and 0. 1% magnesium sulfate. The major factors in laboratory shake flask liquid fermentation,such as initial p H,temperature,speed,etc were determined. The optimum cultural conditions are found to be incubation time 20 h,p H7. 5,fermentation temperatures 30 ℃,speed 200 r·min- 1,inoculum size 3%,and medium volume 35 m L in a 250 m L flask. The OD600 value of the bacterial growth was increased by 21. 9% after optimization of the cultural conditions.

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