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当前位置: 首页 > 文章 > 噬菌体对荧光假单胞菌A46的感染及其防治的初步研究 食品科学 2009,30 (5) 197-200
Position: Home > Articles > Preliminary Study on Bacteriophage Infection of Pseudomonas fluorescens A46 and Corresponding Control FOOD SCIENCE 2009,30 (5) 197-200

噬菌体对荧光假单胞菌A46的感染及其防治的初步研究

作  者：
张志平;李晓明;孙文敬;王多;刘敬泽
单  位：
河北师范大学生命科学学院
关键词：
荧光假单胞菌;2-酮基-D-葡萄糖酸;噬菌体;感染;菌体形态
摘  要：
利用透射电镜观察2-酮基-D-葡萄糖酸产生菌荧光假单胞菌A46感染噬菌体KS461-2后的菌体形态变化,测定该菌株耐噬菌体突变频率,考察不同pH值对噬菌体增殖的影响,检测4种常用药物对噬菌体的防治效果。结果表明,随着噬菌体感染时间的延长,菌体形态变化差异显著;该菌株耐噬菌体KS461-2的突变频率在2.5×10-6左右;噬菌体在pH7.5～8.0的条件下容易吸附于宿主而增殖;草酸铵能有效抑制该噬菌体的增殖,而对宿主菌的生长无明显影响,最佳添加浓度为0.7%。
译  名：
Preliminary Study on Bacteriophage Infection of Pseudomonas fluorescens A46 and Corresponding Control
作  者：
ZHANG Zhi-ping1,LI Xiao-ming1,SUN Wen-jing1,2,WANG Duo1,LIU Jing-ze1, (1. College of Life Sciences, Hebei Normal University, Shijiazhuang 050016, China ; 2. College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China)
关键词：
Pseudomonas fluorescens;2-keto-D-gluconic acid;bacteriophage;infection;cell morphology
摘  要：
The changes of cell morphology of Pseudomonas fluorescens A46, a strain producing 2-keto-D-gluconic acid, were observed by transmission electron microscope after infected by bacteriophage KS461-2. The mutation frequency of P. fluorescens A46 resisting the bacteriophage was determined, the multiplication of the bacteriophage was studied at different pH values, and the control effects of four medicaments on the bacteriophage infection were investigated. The current results showed that the morphology significantly changes following with infection time extending, the mutation frequency of P. fluorescens is about 2.5×10-6, the bacteriophage easily adheres to the host bacteria and proliferates in the pH range of 7.5 to 8.0, and ammonium oxalate can remarkably restrain the multiplication of the bacteriophage and does not affect the growth of P. fluorescens A46, especially the addition of 0.7% is the best.

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