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当前位置: 首页 > 文章 > 吡嘧磺隆对稻田土壤真菌群落结构及土壤酶活性的影响 水土保持研究 2016,23 (2) 73-77
Position: Home > Articles > Effects of Pyrazosulfuron-ethyl on Soil Fungal Community Structure and Enzyme Activity Research of Soil and Water Conservation 2016,23 (2) 73-77

吡嘧磺隆对稻田土壤真菌群落结构及土壤酶活性的影响

作  者：
曹焜;荆瑞勇;周颖;王光华;郭永霞
单  位：
中国科学院东北地理与农业生态研究所黑土区农业生态重点实验室;黑龙江八一农垦大学
关键词：
微生物生态学;真菌群落结构;土壤酶;吡嘧磺隆
摘  要：
采用PCR-DGGE和比色法,在室内培养条件下研究施用不同浓度吡嘧磺隆(0.4,4,40mg/kg)对土壤真菌群落结构及土壤酶活性的影响。结果表明:施用吡嘧磺隆7d时提高了土壤脱氢酶及多酚氧化酶活性,抑制葡聚糖酶活性,真菌多样性指数无明显变化;14d时,随着施药浓度的增大,脱氢酶活性减弱,多酚氧化酶活性增强,真菌多样性指数逐渐增大。21d时,随施用吡嘧磺隆浓度增大,土壤脱氢酶和多酚氧化酶活性会降低,而土壤葡聚糖酶恢复至对照水平,真菌多样性指数增大。试验结果为全面了解吡嘧磺隆对土壤微生态环境的影响提供数据依据。
译  名：
Effects of Pyrazosulfuron-ethyl on Soil Fungal Community Structure and Enzyme Activity
作  者：
CAO Kun;JING Ruiyong;ZHOU Ying;WANG Guanghua;GUO Yongxia;Heilongjiang Bayi Agricultural University;Key Laboratory of Mollisols Agroecology,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences;
关键词：
microbial ecology;;diversity of fungi;;soil enzyme;;pyrazosulfuron-ethyl
摘  要：
Effects of different concentrations of pyrazosulfuron-ethyl(0.4,4,40mg/kg)on fungal community structure and soil enzyme activity were investigated by PCR-DGGE and colorimetric methods.The results showed that pyrazosulfuron-ethyl can promote the activities of soil dehydrogenase and polyphenol oxide,inhibit the activity of glucanase and have no influence on fungal community structure at 7th day At 14 th day,the activity of dehydrogenase decreased and activity of polyphenol oxide increased,and the diversity index of fungal community increased gradually with increase of pyrazosulfuron-ethyl concentration.At 21 th day,the activities of dehydrogenase and polyphenol oxide become smaller,but the activity of glucanase recover to the level of control and diversity index of fungal community structure increases gradually with increase of the pyrazosulfuron-ethyl concentration.The above results offered the data support for entirely investigating the effect of pyrazosulfuron-ethyl on soil micro-ecology environment.

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