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Position: Home > Articles > Extraction,separation and purification of chondroitin sulfate from chicken keel cartilage Transactions of the Chinese Society of Agricultural Engineering 2009,25 (1) 271-275

鸡胸软骨硫酸软骨素的提取及分离纯化

作  者:
熊双丽;李安林;吴照民;魏明
单  位:
西南科技大学生命科学与工程学院
关键词:
提取;纯化;分离;鸡胸软骨;硫酸软骨素;分离纯化
摘  要:
为优化硫酸软骨素的提取工艺和初步分析其结构,采用中心组合设计和响应面分析优化鸡胸软骨硫酸软骨素的酶法提取工艺,三氯乙酸除蛋白质、乙醇沉淀以及柱色谱精细纯化得到高纯度硫酸软骨素,并采用高效凝胶过滤色谱判定其纯度,红外光谱和核磁共振初步分析其结构。结果表明,硫酸软骨素的最佳提取工艺参数为温度55.68℃,pH值为6.40,时间为3.85h,葡萄糖醛酸和硫酸软骨素提取率分别达到9.82%和28.05%。高效凝胶过滤色谱分析其纯度为99.01%。红外光谱和核磁共振显示硫酸软骨素主要是4硫酸化软骨素。
译  名:
Extraction,separation and purification of chondroitin sulfate from chicken keel cartilage
作  者:
Xiong Shuangli,Li Anlin,Wu Zhaomin,Wei Ming (School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,China)
关键词:
extraction,purification,seperation,chicken keel cartilage,chondroitin sulfate
摘  要:
This article mainly adopted response surface methodology to optimize the extracting technology of chondroitin sulfate by enzyme.Chondroitin sulfate with high purity was obtained after trichloacetic acid for deproteinization,alcohol precipitation and column chromatography for further isolation and purification.High performance gel filtration chromatography was used to identify purity of chondroitin sulfate.Infrared spectrum and nuclear magnetic resonance were used to analyze its isomerism.The results show that the optimal condition of extraction was 55.68℃of temperature,6.40 of pH and 3.85 h of extraction time.The extraction rate of glucuronic acid and chondroitin sulfate is 9.82%and 28.05% respectively.The data of high performance gel filtration chromatography exhibited that obtained chondroitin sulfate is of highly purity(99.01%).Infrared spectra and nuclear magnetic resonance exhibited that it is chondroitin-4-sulfate.

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