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Position: Home > Articles > Proficiency Testing for Determination of Deoxynivalenol in Corn Flour Journal of Anhui Agricultural Sciences 2022,50 (1) 177-179+223

玉米粉中脱氧雪腐镰刀菌烯醇含量的测定能力验证

作  者:
王蓓蓓;朱联旭;李崇勇;刘欣
单  位:
镇巴县市场监督管理局;陕西理工大学生工学院;西安市质量与标准化研究院;汉中市食品药品监督检验检测中心
关键词:
脱氧雪腐镰刀菌烯醇;能力验证;玉米粉;高效液相色谱法
摘  要:
[目的]通过实验室能力验证活动分析了影响玉米粉中脱氧雪腐镰刀菌烯醇(DON)检测结果的主要因素。[方法]依据GB 5009.111—2016第二法:免疫亲和层析净化HPLC法检验并优化前处理条件。[结果]采用5倍稀释浓度提取1 h和3 mL/min的净化速度,能力验证样品中DON含量为571μg/kg, RSD为0.25%。[结论]通过了中国食品药品检定研究院组织实施的NIFDC-PT-228《玉米粉中脱氧雪腐镰刀菌烯醇的测定》能力验证,与中国食品药品检定研究院统计Z比分值为0.70,能力验证结果为满意,细化完善了国标测定脱氧雪腐镰刀菌烯醇的操作,为广大检验检测人员提供参考。
译  名:
Proficiency Testing for Determination of Deoxynivalenol in Corn Flour
作  者:
WANG Bei-bei;ZHU Lian-xu;LI Chong-yong;Hanzhong Testing and Inspection Center for Food and Drug Control;College of Biological Science and Engineering, Shaanxi University of Technology;Zhenba County Market Supervision Administration;
关键词:
Deoxynivalenol;;Proficiency testing;;Corn flour;;High performance liquid chromatography
摘  要:
[Objective]Through a laboratory proficiency testing activity, the main factors affecting the detection results of deoxynivalenol in corn flour were analyzed. [Method]According to the second method of the national standard GB5009.111-2016 to check and optimize the pretreatment conditions.[Result]Under the conditions of extraction at 5 times of dilution concentration for 1 h and 3 mL/L flow rate, the DON content in the ability verification sample was 571 μg/kg, RSD was 0.25%.[Conclusion]Passed the NIFDC-PT-228 “Deoxynivalenol Determination of Deoxynivalenol in Corn Flour” proficiency test organized and implemented by the China Institute for Food and Drug Control, and the Z score with the organizer was 0.70. The proficiency test results were satisfactory, and the national standard determination of deoxynivalenol has been refined and perfected to provide a reference for the majority of inspection and testing personnel.

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