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Position: Home > Articles > Optimization of Maillard Reaction Conditions of Fructose and Hydroxyproline Journal of Henan Agricultural Sciences 2014,43 (4) 147-151

果糖与羟脯氨酸美拉德反应条件的优化

作  者:
程传玲;杨艳勤;宋辉;王建民
单  位:
郑州轻工业学院食品与生物工程学院
关键词:
果糖;羟脯氨酸;美拉德反应;气相色谱-质谱分析
摘  要:
为确定果糖与羟脯氨酸进行美拉德反应的最佳条件,以美拉德反应产物在420nm下的吸光值为评价指标,通过单因素试验和正交试验,研究了反应温度、反应时间、初始pH值、外加水分、糖氨配比对果糖与羟脯氨酸美拉德反应产物质量的影响。结果表明,最佳反应条件为:反应温度160℃、反应时间3h、初始pH值8、糖氨摩尔比1∶3、外加水分0,该条件下美拉德反应产物的吸光值为0.835。对美拉德反应产物进行GC-MS分析,鉴定出15种挥发性香气成分,其主要为吡嗪、吡咯、呋喃等杂环化合物,表明果糖与羟脯氨酸的美拉德反应产物具烟草增香作用。
译  名:
Optimization of Maillard Reaction Conditions of Fructose and Hydroxyproline
作  者:
CHENG Chuan-ling;YANG Yan-qin;SONG Hui;WANG Jian-min;School of Food and Biological Engineering,Zhengzhou University of Light Industry;
关键词:
fructose;;hydroxyproline;;Maillard reaction;;GC-MS
摘  要:
Using absorbance value of Maillard reaction products in 420nm as the evaluation index, through the single factor experiment and orthogonal test,the effects were studied of reaction temperature,reaction time,initial pH value,additional water and sugar ammonia ratio on the product quality of fructose and hydroxyproline Maillard reaction.The results showed that the optimal reaction condition was:reaction temperature of 160 ℃,reaction time of 3h,initial pH value of 8,sugar ammonia molar ratio of 1∶3,without moisture.Under the best condition the absorption value of Maillard reaction products was 0.835.GC-MS analysis of Maillard reaction products identified 15 kinds of volatile aroma components,and the main components were pyrazine,pyrrole,furan and other heterocyclic compounds.The results showed that the Maillard reaction products of fructose and hydroxyproline could improve the flavor of tobacco.

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