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当前位置: 首页 > 文章 > 毛细管电泳电化学检测法测定枣中糖类物质 食品科学 2003,24 (12) 91-94
Position: Home > Articles > Simultaneous Assay on Sucrose Glucose and Fructose in Jujubeby Capillary Electrophoresis with Electrochemical Detection FOOD SCIENCE 2003,24 (12) 91-94

毛细管电泳电化学检测法测定枣中糖类物质

作  者：
傅崇岗;单瑞峰;苏昌华
单  位：
聊城大学化学化工学院
关键词：
毛细管电泳;枣;蔗糖;葡萄糖;果糖
摘  要：
采用高效毛细管电泳—电化学检测法同时测定了枣中的主要成分蔗糖、葡萄糖、果糖的含量,在15min内可实现三种组分的基线分离;研究了电极电位,电解液的浓度,电泳电压和进样时间等对分析性能的影响,得到了最优分离检测条件。以铜圆盘电极为工作电极,工作电极电位为0.75V(vsAg/AgCl),电泳介质为0.1mol/LNaOH溶液。在0.002～2mmol/L的浓度范围内,蔗糖、葡萄糖和果糖果线性范围均为0.002～2mmol/L。检出限分别为0.78、0.46、0.71μmol/L(3倍 S/N)。峰电流的相对标准偏差RSD分别为2.13%、1.22%和0.26%(n=7,c=0.4mmol/L)。
译  名：
Simultaneous Assay on Sucrose Glucose and Fructose in Jujubeby Capillary Electrophoresis with Electrochemical Detection
作  者：
FU Chong-gang,SHAN Rui-feng,SU Chang-hua(School of Chemisty and Chemical Engineering, Liaocheng University, Liaocheng 252059, China)
关键词：
capillary eletrophoresis;jujube;sucrose;glucose;fructose
摘  要：
As main ingredients, sucrose, glucose and fructose in jujube could be simultaneously determined by capillaryelectrophoresis with electrochemical detection. The three species could be well separated within 15min under optimizedconditions. The effects of detection potential, concentration of electrolyte, separation voltage and injection time on the analyticalcharacterization were investigated. A copper electrode was used as the working electrode with the detection potential of +0.75V(vs. Ag/AgCl). The solutions of 0.1 mol/L NaOH were chosen as the electrophoretic media. Good linear relationship betweenpeak current and concentration existed in the range of 0.002～2 mmol/L for the three analytes studied. Good sensitivity andprecision was obtained under the optimized conditions with the detection limits of 0.78、0.46、0.71μmol/L(3 times S/N)andpeak height RSDs(n=7,c=0.4mmol/L) of 2.13%,1.22%,0.26% for sucrose, glucose and fructose respectively.

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