中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 饲料浮萍水平对大正三色锦鲤TYR及MC1R基因表达的影响 水产科学 2017,36 (2) 172-177
Position: Home > Articles > Effects of Dietary Duckweed Levels on Expression of Tyrosinase Gene and Melanin Cortisol Receptors Gene in Koi Tajsho-sanke Fisheries Science 2017,36 (2) 172-177

饲料浮萍水平对大正三色锦鲤TYR及MC1R基因表达的影响

作  者：
张植元;魏东;乔秀亭;白东清;范泽;李静辉;安贸麟
单  位：
天津农学院水产学院天津市水产生态及养殖重点实验室
关键词：
浮萍;大正三色锦鲤;TYR基因;MC1R基因;黑色素
摘  要：
为探究饲料中不同浮萍添加水平对大正三色锦鲤酪氨酸酶TYR以及黑素皮质素受体MC1R基因表达的影响,配制浮萍添加水平分别为0%、3%、6%、9%、13%、14%6种等蛋白(38.6%)饲料饲养大正三色锦鲤(42.19±1.32)g 8周,分别记为Diet 1(0/13)、Diet 2(3/10)、Diet 3(6/7)、Diet 4(9/4)、Diet 5(13/1)、Diet 6(14/0)。每种饲料设3个重复,每个重复饲喂10尾鱼。试验结果表明:(1)TYR基因在大正三色锦鲤的心脏、肝脏、眼睛、黑色皮肤、脑中均有表达,并且在脑、皮肤、眼睛中表达量相对较高;(2)各组织中TYR mRNA表达量随浮萍添加水平的升高均有一定波动,在皮肤与眼睛中,以Diet 6(14/0)组显著高于其他各组(P<0.05),在脑中,以Diet 3(6/7)组显著高于除Diet 6的其他各组(P<0.05);(3)皮肤与眼睛中MC1R mRNA表达量以Diet 6(14/0)组显著高于其他各组(P<0.05),而在脑中各组差异均不显著(P>0.05)。综上所述,在本试验条件下,饲料不同浮萍添加水平促进大正三色锦鲤TYR以及MC1R基因表达,有利于鱼体黑色素沉积,因此选择Diet 6(14/0)组饲料投喂大正三色锦鲤最适。
译  名：
Effects of Dietary Duckweed Levels on Expression of Tyrosinase Gene and Melanin Cortisol Receptors Gene in Koi Tajsho-sanke
作  者：
ZHANG Zhiyuan;WEI Dong;QIAO Xiuting;BAI Dongqing;FAN Ze;LI Jinghui;AN Maolin;Department of Fishery Science,Tianjin Agricultural University;
关键词：
duckweed;;koi Tajsho-sanke;;TYR gene;;MC1Rgene;;melanin pigment
摘  要：
The present study was conducted to investigate the effect of dietary duckweed levels on the expression of tyrosinase(TYR)gene and melanin cortisol receptors gene(MC1R)in koi Tajsho-sanke(Cyprinus carpio).Six isonitrogenous diets(38.6%)formulated ocontained 0%,3%,6%,9%,13%,and 14%duckweed replacing rapeseed meal[referred to as Diet 1(0/13),Diet 2(3/10),Diet 3(6/7),Diet 4(9/4),Diet 5(13/1)and Diet 6(14/0)].A total of 180 fish with body weight of(42.19±1.32)g were randomly divided into five groups with three replicates and fed twice a day(09:00and 15:30)for 8weeks.The results showed that TYR mRNA was detected in heart,hepatopancreas,eye,black skin and brain tissue of Tajsho-sanke,with higher TYR expression in the brain,black skin and eye.With the increase in duckweed level,the expression quantity of tyrgene showed some fluctuation.There were significantly higher expression of tyrgene in eye and black skin in Diet 6(14/0)than in the other groups(P<0.05),while the expression quantities were significantly higher in brain in Diet 3(6/7)compared with other groups(P<0.05).The expression quantities of MC1 Rin eye and black skin of the fish in Diet 6(14/0)were significantly higher than those in other goups(P<0.05).There was no significant difference in MC1 Rexpression in brain among all groups(P>0.05).The findings indicate that 14% duckweed level can promote TYR and MC1 Rexpression and improve melanin deposition.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46