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Position: Home > Articles > Study on the liquid fermentation conditions of glucoamylase by Monascus purpureus Science and Technology of Food Industry 2017 (14) 146-150+155

紫色红曲霉液态发酵产糖化酶的工艺研究

作  者:
陈景智;林娟;叶秀云;徐美爱;李雯娟;邓香圳
单  位:
福州大学福建省海洋酶工程重点实验室
关键词:
紫色红曲霉;糖化酶;培养基;发酵条件
摘  要:
通过培养基和发酵条件的优化提高紫色红曲霉G6液态发酵产糖化酶活力。研究接种菌龄、碳源、氮源、金属离子、培养基初始pH、接种量、装液量、摇床转速以及温度等对产酶的影响。结果表明,最佳培养基配方(w/v)为大米粉8%,蛋白胨1%,KCl0.1%,ZnSO_40.01%,FeSO_40.005%,MnSO40.015%,培养基初始pH为4.0;最适发酵条件:装液量50mL/250mL,转速150r/min,温度32℃,接种量12%(v/v);在此条件下发酵10d,糖化酶活力为1652.36U/mL比优化前(109.54U/mL)提高了14.08倍。
译  名:
Study on the liquid fermentation conditions of glucoamylase by Monascus purpureus
作  者:
CHEN Jing-zhi;LIN Juan;YE Xiu-yun;XU Mei-ai;LI Wen-juan;DENG Xiang-zhen;Fujian Key Laboratory of Marine Enzyme Engineering,Fuzhou University;
关键词:
Monascus purpureus;;glucoamylase;;culture medium;;fermentation conditions
摘  要:
The production conditions of glucoamylase through liquid-state fermentation by Monascus purpureus G6 were optimized.Based on results of single factor tests,the optimal conditions for glucoamylase production were 8% rice powder,1%peptone,0.1% KCl,0.01% ZnSO_4,0.005% Fe SO4,0.015% MnSO_4,50 m L/250 m L loading medium volume( v/v),12%inoculation amount( v/v) and rotation speed of shaking incubator was 150 r/min,initial p H value of culture medium was 4.0,fermentation temperature was 32 ℃.The fermentation was carried out under these conditions for 10 days to obtain the maximum glucoamylase activity of 1652.36 U/m L,which was 14.08 times higher than that without optimization of fermentation condition.

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