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Position: Home > Articles > The Ligation of Gene xanA in Xanthomonas campestris and pUCm-T Vector FOOD SCIENCE 2006,27 (11) 171-173

野油菜黄单胞菌xanA基因与pUCm-T载体的连接

作  者:
袭凌;李柏林;欧杰
单  位:
上海水产大学食品学院
关键词:
野油菜黄单胞菌;xanA基因;pUCm-T载体;连接
摘  要:
本文研究了两种不同的连接方法在野油菜黄单胞菌(Xanthomonascampestris)xanA基因与pUCm-T载体连接中的连接效果,并进行了转化实验进行鉴定。实验结果表明,xanA基因与pUCm-T载体的适宜连接条件为16℃连接过夜。连接体系中,xanA基因与pUCm-T载体的体积比为4:1。
译  名:
The Ligation of Gene xanA in Xanthomonas campestris and pUCm-T Vector
作  者:
XI Ling,LI Bai-lin,OU Jie* (College of Food Science,Shanghai Fisheries University,Shanghai 200090,China)
关键词:
Xanthomonas campestris;gene xanA;pUCm-T vector;ligation
摘  要:
Two methods for gene xanA to ligate with pUCm-T vector were discussed in this thesis. And the transformations were also done for testing. It showed that the better ligation condition was ligated at 16℃ for overnight. In the ligation system,the volume ratio of gene xanA to pUCm-T vector was 4:1.

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