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Position: Home > Articles > Comparative Study of Determination Methods for Glycine in Foods FOOD SCIENCE 2012,33 (18) 254-257

液相色谱法与氨基酸分析法测定食品中甘氨酸的比较研究

作  者:
刘小力
单  位:
国家食品质量安全监督检验中心
关键词:
甘氨酸;液相色谱;氨基酸分析
摘  要:
建立对食品中游离甘氨酸经提取、净化等处理后,邻苯二甲醛衍生,经高效液相色谱测定分析,并将该方法与氨基酸分析仪测定甘氨酸的方法进行比较研究。通过对市售的液体乳、含乳饮料(品)类和植物蛋白饮料(品)类中游离甘氨酸分别应用两种方法进行测定,结果表明,氨基酸分析仪法测定甘氨酸具有测定结果稳定准确、检出限低和操作简单的特点,而液相色谱法(荧光检测器)测定甘氨酸检测时间快,检出限也能满足常规检测的需要,因此,在实际应用中,两种方法均可采用,可作为互相验证的方法。
译  名:
Comparative Study of Determination Methods for Glycine in Foods
作  者:
LIU Xiao-li(National Food Quality & Safety Supervision and Inspection Center,Beijing 100094,China)
关键词:
glycine;HPLC;amino acid analysis
摘  要:
Glycine,as a non-essential amino acid in the human body,has the simplest structure among amino acid series.Glycine is widely used as a food additive with the maximum level of 1.0 g/kg in the food industry.Recent years,industrial grade glycine is illegally used in food products to substitute milk or improve protein content.In this paper,an HPLC method was established for the determination of glycine and compared with the amino acid analyzer method.Meanwhile,glycine contents in commercial milk,milk drink and plant protein products were determined by the HPLC method and the amino acid analyzer method.The results indicated that the amino acid analyzer method had advantages of stable and accurate results,low detection limit and simple operation procedures,while the HPLC method had the advantages of time saving and enough low detection limit compared with the routine fluorescence detection.Therefore,both methods can be applied for practical determination of glycine in food products as mutual authentication methods.

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