当前位置: 首页 > 文章 > 丽蝇蛹集金小蜂寄生棕尾别麻蝇的生理学效应 浙江大学学报(农业与生命科学版) 2005,31 (1)
Position: Home > Articles > Physiological effects of parasitization by Nasonia vitripennis on its host pupae of Boettcherisca peregrina. Journal of Zhejiang University (Agriculture and Life Sciences) 2005,31 (1)

丽蝇蛹集金小蜂寄生棕尾别麻蝇的生理学效应

作  者:
张焘;张忠;李凯;叶恭银;胡萃
单  位:
浙江大学应用昆虫学研究所
关键词:
丽蝇蛹集金小蜂;棕尾别麻蝇;寄生;血细胞;血淋巴;可溶性蛋白;可溶性糖
摘  要:
丽蝇蛹集金小蜂Nasoniavitripennis寄生能引起寄主棕尾别麻蝇Boettcheriscaperegrina蛹的血细胞数量、血淋巴中可溶性蛋白组成与含量,及可溶性糖含量发生明显的变化.寄生蛹的血细胞总数、浆血细胞和颗粒细胞数量都极显著多于未寄生蛹,而血淋巴中可溶性蛋白和糖的含量则均极显著低于未寄生蛹.SDS-PAGE电泳结果表明,寄生蛹与未寄生蛹间血淋巴可溶性蛋白电泳图谱有一定的差异,其中分子量为28kD、32kD和100kD蛋白谱带的丰度自寄生2d后起多明显弱于未寄生蛹,而分子量约14kD的蛋白谱带则恰相反.
译  名:
Physiological effects of parasitization by Nasonia vitripennis on its host pupae of Boettcherisca peregrina.
作  者:
ZHANG Tao, ZHANG Zhong, LI Kai, YE Gong-yin, HU Cui (Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China)
关键词:
Nasonia vitripennis; Boettcherisca peregrina; parasitism; hemocyte; hemolymph; soluble protein; soluble sugar
摘  要:
The parasitization by Nasonia vitripennis (Hymenoptera: Pteromalidae) could result in marked changes in hemocyte population and composition and level of soluble proteins as well as the soluble sugar level in the hemolymph of Boettcherisca peregrina pupae. Total hemocyte counts and the counts of plasmatocyte and granulocyte were significantly larger in parasitized pupae than in unparasitized ones, while both levels of soluble proteins and soluble sugars in the hemolymph of parasitized pupae were found to be clearly lower than that in unparasitized ones. The SDS-PAGE electrophoresis showed some differences in the electrophoresis profile of hemolymph soluble proteins between parasitized and unparasitized pupae. The richness of three protein bands with molecular weight of 28 kD, 32 kD and 100 kD in parasitized pupae was significantly lower than that in unparasitized ones since the 2nd day after parasitism. The reverse was true for another protein band with molecular weight of about (14 kD.)

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