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Position: Home > Articles > Establishment of Rapid In-vitro Propagation System of Liriodendron tulipifera Journal of Zhejiang Forestry Science and Technology 2009,29 (6) 29-31

北美鹅掌楸组培快繁技术体系的建立

作  者:
陈颖;周统建;曹福亮;桂仁意;周吉玲
单  位:
浙江林学院;南京林业大学
关键词:
北美鹅掌楸;组织培养;快繁
摘  要:
通过北美鹅掌楸(Liriodendron tulipifera)种子诱导的无菌苗茎段,建立北美鹅掌楸的组培快繁体系,种子接种10 d后开始萌动,实验的4种培养其中,最好的培养基是MS+3%蔗糖,种子萌发率达38.4%;MS+6-BA1.0 mg/L+NAA0.1 mg/L是最好的外植体分化培养基,其增值率达2.04倍;小芽丛在MS+6-BA0.5 mg/L+NAA0.05 mg/L上外植体分化数最高,达2.45倍,芽苗生长较好;芽苗在1/2MS+IBA0.5 mg/L+NAA0.1 mg/L生根培养基上,13 d后开始生根,生根率最高达66.7%,炼苗移栽20 d后苗木成活率达80%以上。
译  名:
Establishment of Rapid In-vitro Propagation System of Liriodendron tulipifera
作  者:
CHEN Yin1,ZHOU Tong-jian1,CAO Fu-liang1,GUI Ren-yi2,ZHOU Ji-ling1(1.College of Forest Resources and Environment,Nanjing Forestry University,Najing 210037,China;2.School of Forestry and Biotechnology,Zhejiang Forestry College,Lin’an 311300,China)
关键词:
Liriodendron tulipifera;tissue culture;rapid propagation
摘  要:
Experiment was conducted for establishment of rapid propagation system of Liriodendron tulipifera by stem of non-vaccine-induced seed.The result showed that seed germination started 10 days after inoculation and germination rate reached 38.4% in MS+3% of sucrose.MS+ 6-BA1.0mg/L+NAA0.1mg/L was the best medium for explant to differentiation and coefficient of multiplication was 2.04.MS+6-BA0.5mg/L+ NAA0.05mg/L was the most appropriate medium for sprouts and the proliferation topped to 2.45.The best medium for rooting was 1/2MS+ IBA0.5mg/L+NAA0.1mg/L,and the highest rooting rate attained to 66.7%.20 days after hardening and transplanting,survival rate of seedlings reached more than 80%.

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