The effect of rhG-CSF on blood physiological indexes and histomorphology of breast in Kunming mice with E. coli mastitis

LI Lang;WAN Tao-mei;YUAN Gui-qiang;ZUO Zhi-cai;WANG Zheng-yi;REN Yi;Key Laboratory of Animal Disease and Human Health of Sichuan Province/Key Laboratory of Environmental Hazard and Animal Disease of Sichuan Province/College of Veterinary Medicine,Sichuan Agricultural University;

granulocyte colony-stimulating factor;;E;;coli;;mice;;mastitis;;protective effect

To explore recombinant granulocyte colony-stimulating factor(rhG-CSF)on the prevention effect of mastitis,mammary 8-10 days 120 mice were randomly divided into five groups.Ⅰ,Ⅱgroup of subcutaneous injection of 0.1 mL saline;groupⅢ,ⅣandⅤ,respectively injection by 10,20 and 30μg/kg bodyweight 0.1 mL rhG-CSF.After injecting 24 h,theⅠ group was given 50μL normal saline,and the Ⅱ,Ⅲ,Ⅳ and Ⅴ were given 50μL E.coli at concentration of 1.5×107 CFU/mL.After infection in 0,24,72,120 h,sampling of randomly selected 6 mice in each group,to detect the contents of blood serum TNF-α,the number of E.coli and histopathological changes of mammary gland.The results showed that total number of white blood cells in the blood and classification decreased,theⅤ group was significantly higher than that of groupⅠ,Ⅱ,Ⅲ,Ⅳ;comparison of serum TNF-αcontent at 24 h,Ⅱ group was significantly higher than theⅠ,Ⅲ,Ⅳ and Ⅴgroup,at 72 and 120 h,Ⅱ,Ⅲ,Ⅳ andⅤ group was significantly higher than group Ⅰ;at 20 h,Ⅴgroup without detection of E.coli,and no obvious inflammation of the breast tissue in groupⅡthe number of E.coli was significantly higher than that of group Ⅲ and Ⅳ,Ⅱ group mice mammary gland cavity and stroma were observed a large number of inflammatory cells infiltration,disorder of organizational structure,groupⅢ had a small amount of inflammatory cells infiltration,Ⅳ group structure full,injury minor.The rhG-CSF by increasing the number of white blood cells in the blood of mice improves the clearance rate of bacteria,so as to reduce tissue damage,and 30μg/kg to best effect.