单 位：
河北北方学院基础医学院解剖教研室;河北北方学院基础医学院组胚教研室
关键词：
粒细胞集落刺激因子;脑挫伤;血管紧张素Ⅱ;内皮素;大鼠
摘 要：
目的探讨粒细胞集落刺激因子(G-CSF)对脑挫伤大鼠血浆及脑组织中血管紧张素Ⅱ(AngⅡ)和内皮素(ET)含量的影响.方法采用自由落体法致SD大鼠脑挫伤动物模型.将大鼠随机分为治疗组、对照组和正常组,治疗组脑挫伤后2h开始应用G-CSF(浓度为2mg/L)皮下注射10μg/kg/d,共5d.对照组和正常组2h后给予等量的生理盐水皮下注射,共5d.正常组假手术不致伤.每组大鼠再随机分为两个亚组分别为治疗1周组、治疗2周组、对照1周组、对照2周组、正常1周组和正常2周组.分别在造模成功1周和2周后检测各组血浆及脑组织中AngⅡ和ET的含量.结果 1周:对照组血浆及脑组织中AngⅡ和ET的含量均增高,与正常组比较有差异(P<0.05).2周:对照组血浆及脑组织中AngⅡ和ET的含量增加更加明显,与正常组比较有显著的差异(P<0.01).治疗组1周和2周的结果是血浆及脑组织中AngⅡ和ET的含量与对照组比较均有降低,差异有统计学意义(P<0.05).结论脑挫伤大鼠血浆及脑组织中AngⅡ和ET的含量明显升高,G-CSF能降低两者的含量,从而起到对受损伤的脑组织的保护作用.
译 名：
Effects of G-CSF on AngⅡ and ET in Blood Plasma and Brain Tissue after Brain Contusion in Rats
作 者：
SUN Lin1,ZHANG Wen-jing2(Basic Medical College,Hebei North University,Zhangjiakou 075000,Hebei,China)
关键词：
G-CSF;brain contusion;AngⅡ;ET;rat
摘 要：
Objective To investigate the influence of granulocyte colony stimulating factor(G-CSF)on angiotensin Ⅱ(AngⅡ)and endothelin(ET)content in plasma and brain tissue of brain contusion rats.Methods SD rat models of brain contusion established by free fall method were divided ramdomly into 3 groups including treatment group,control group and normal group.Injected rhG-CSF which was diluted with normal saline(the concentration is 2 mg/L) 10 ug/kg/d after treatment group model had been completed 2 hours for 5 days.Rats of control group and normal group were injected the same amount of saline 2 hours later for 5days.Rats of Normal group were not hurt.Rats of each group were randomly divided into two subgroups:1 W,2 W treatment groups,1 W,2 W control groups,1 W and 2 W normal groups.The content of AngⅡand ET in plasma and brain tissue of every group was tested after completing the model one and two weeks.Results In comparison with that of normal group,AngⅡand ET were both increased significantly in control group after one week(P<0.05).After 2 weeks AngⅡand ET were both increased more significantly in control group in comparison with that of normal group(P<0.01).Compared with that of control group,AngⅡ and ET in the plasma and brain tissue decreased significantly in treatment group(P<0.05).Conclusion G-CSF can increase the content of AngⅡ and ET in brain contusion rat plasma and brain tissue,G-CSF has a protective effect on the injured brain tissue.
