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Position: Home > Articles > Study on the Acidic Polysaccharides of Zizyphus Jujube MILL FOOD SCIENCE 2004,25 (5) 55-58

大枣酸性多糖ZJ-6的化学研究

作  者:
杨云;孟江;冯卫生;田润涛
单  位:
河南中医学院药学院
关键词:
大枣;多糖;分离纯化;结构测定
摘  要:
大枣用乙醇回流脱脂,80~100℃温水浸取,提取液减压浓缩,乙醇分级沉淀,三氯乙酸脱蛋白,透析,透析内液减压浓缩,再醇沉得到三个级分ZJ-A,ZJ-B,ZJ-C,ZJ-B经DEAE-Cellulose柱、sepchcryls-300柱分离纯化,得大枣多糖ZJ-6,HPGPC法测定其分子量为4206,光谱和色谱分析(1HNMR,13CNMR,HPLC)表明,ZJ-6是以α-D-(1→4) -半乳糖醛酸为主链,葡萄糖、木糖、阿拉伯糖在末端或支链相连的酸性杂多糖。
译  名:
Study on the Acidic Polysaccharides of Zizyphus Jujube MILL
作  者:
YANG Yun,MENG Jiang,FENG Wei-sheng,TIAN Run-tao(Henan College of Traditional Chinese Medicine,Zhengzhou 450008,China)
关键词:
Zizyphus jujuba MILL;isolation;purification;structure
摘  要:
Zizyphus jujuba MILL was extracted with ethanol and 80~100℃water, the water elution was concentrated at reducepressure, fractions ZJ-A,ZJ-B,ZJ-C were obtained by Fractional precipitation of the water elution with ethanol.And thenprotein was cleared by CCl3COOH. While ZJ-6 was obtained by further purification of ZJ-B with DEAE -Cellulose and Sepchcryls-300. The molecular weight of ZJ-6 was estimated to be 4206 with HPGPC. Spectral and chromatographic analysis (1HNMR,13CNMR,HPLC) were used to investigated the structural characterization of the polysaccharide.Results showed that ZJ-6'mainchain was (1→4)-linked α-D-galacturonic acid ,and it's side chains included glucose、xylose、arabinose.

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