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药用植物研究(英文版)
2010,1
(10)
Position: Home > Articles > Study on the Extraction Process of Total RNA from Dioscorea opposita Leaves
Medicinal Plant
2010,1
(10)
Study on the Extraction Process of Total RNA from Dioscorea opposita Leaves
作 者：
Xiaojuan Zhang;HU Xuan-pin
单 位：
School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shanxi 723001, Chin;School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shanxi 723001, China
关键词：
total rna;leaves;dioscorea opposita;modified ctab method;extracting;isothiocyanat
摘 要：
[Object] To extract high quality total RNA from Dioscorea opposita leaves. [Methods] Leaves of Dioscorea opposite as tested materials, the guanidinium isothiocyanate method and modified CTAB method were introduced for extracting total RNA from Dioscorea opposita leaves, the integrity and purity of total RNA were analyzed by agarose gel electrophoresis and UV-spectrophotometer respectively. [Result] The 28S and 18 SrRNA bands of total RNA extracted by modified CTAB method were clear and visible, A260/A280 was in the range of 1.7-2.0. [Conclusion] Modified CTAB method was suitable for extracting total RNA from Dioscorea opposita Leaves.
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