Yulong Ding;Xiaozhu Liu;Yanhong Li;Hanbin Wang;Gong Xiu-hu

Key Laboratory of Southwest Mountain Forest Resources Conservation and Utilization,Ministry of Education,Southwest Forestry University,Kunming,Yunnan 650224,China;Key Laboratory of Southwest Mountain Forest Resources Conservation and Utilization,Ministry of Education,Southwest Forestry University,Kunming,Yunnan 650224,Chin

total rna;eucommia ulmoides oliv;extracted;bark;modified;metho

[Objective] To extract high quality total RNA from the bark of Eucommia ulmoides Oliv and lay foundation for the isolation of mRNA,cloning of anti-fungi protein gene and library construction of cDNA.[Method] Total RNA from the bark of Eucommia ulmoides Oliv was extracted by modified CTAB-LiCl method,RNApure Plant Kit method and RNAiso Plus method respectively.The quality of total RNA was examined through ultraviolet spectrophotometer and formaldehyde denaturalization agarose gel electrophoresis.[Result] The results showed that total RNA extracted by modified CTAB-LiCl method and RNApure Plant Kit method had higher purity,integrity and yield rate,the ratio of A260/A280 was in the range of 1.8-2.0,the bands of 28S and 18SrRNA were clear;while the quality of total RNA obtained by RNAiso Plus method was poor,the ratio of A260/A280 was 1.652,and the band of RNA was diffusion,which suggested the total RNA was degraded during the extraction process.[Conclusion] The results showed that total RNA from the bark of Eucommia ulmoides Oliv extracted by modified CTAB-LiCl method and RNApure Plant Kit method was of high purity and quality,which could meet the requirement of RT-PCR and RACE etc.experiments as well as lay the foundation for cloning genes from Eucommia ulmoides Oliv.