单 位：
山东农业大学园艺工程学院;山东农业大学林学院
关键词：
毛白杨(Populus tomentosa);PtIAA1;Aux/IAA基因家族;克隆和序列分析
摘 要：
基于NCBI数据库中的序列信息,我们利用PCR技术克隆到一个毛白杨(Populustomentosa)PtI-AA1基因。序列分析表明PtIAA1cDNA长度为946个碱基,编码一个由258个氨基酸组成的蛋白,该蛋白与PtIAA1、NtIAA28和AtIAA16蛋白的同源性分别高达97%、75%和74%。系统进化分析表明PtIAA1基因可能属于Aux/IAA基因家族中组的成员。与大多数的Aux/IAA基因家族中的基因一样,PtIAA1蛋白是一个短命核蛋白,拥有4个高度保守的区域。
译 名：
Cloning and Sequencing of PtIAA1 from Populus tomentosa, a Member of the Aux/IAA Gene Family
作 者：
Dong Xiuchun1 Fan Jinhui1* Yu Xuening1 Shu Huairui2 1 College of Forestry, Shandong Agricultural University, Taian, 271018; 2 College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, 271018
关键词：
Populus tomentosa, PtIAA1, Aux/IAA gene family, Cloning and sequencing
摘 要：
The PtIAA1 gene was amplified from Populus tomentosa based on the sequence information from NCBI database. Sequence analysis indicated that the PtIAA1 cDNA has 964 bp length and encodes a 258-amino acid protein that has 97%, 75% and 74% identity to PtIAA1, NtIAA28 and AtIAA16 respectively. Phylogenic analysis suggests PtIAA1 gene could belong to the group of the Aux/IAA gene family. As same as most of the members of the Aux/IAA gene family, PtIAA1 protein would be a short-lived nuclear protein and also would contain four highly conserved domains
