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Position: Home > Articles > The Rapid Detection of Potato Virus Y by Immunostrip Assay and RT-PCR Hubei Agricultural Sciences 2011,50 (5) 1056-1057+1062

免疫试纸条结合RT-PCR法快速检测马铃薯Y病毒

作  者:
朱云芬;程群;沈艳芬;马作江;王尔惠;魏凯
单  位:
中国南方马铃薯研究中心;湖北省恩施州农业局
关键词:
马铃薯Y病毒;免疫试纸条;RT-PCR
摘  要:
利用马铃薯Y病毒(PVY)免疫试纸条,对PVY进行了快速检测,结果表明,试纸在2 min内,可特异性检出PVY,而健康的叶片无反应。刮下试纸条上显示的检测带,进行RT-PCR,能扩增到与预期大小相同的DNA条带,是对快速检测试纸条检测结果的验证。
译  名:
The Rapid Detection of Potato Virus Y by Immunostrip Assay and RT-PCR
作  者:
ZHU Yun-fen1,CHENG Qun1,SHEN Yan-fen1,MA Zuo-jiang1,WANG Er-hui1,WEI Kai2(1.Southern Potato Research Center of China,Enshi 445002,Hubei,China; 2.Enshi Agriculture bureau,Enshi 445002,Hubei,China)
关键词:
potato virus Y;immunostrip;RT-PCR
摘  要:
Potato virus Y was rapidly detected by immunostrip assay,the results indicated that the PVY could be tested by immunostrip within 2 minutes and no-specific reaction was observerd for healthy leaves.RT-PCR amplification could produce a specific DNA band with an expected size,which was a validation of the results of immunostrip.

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