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Position: Home > Articles > Establishment and Optimization of Salvia przewalskii Maxim. ISSR-PCR Reaction System Journal of Anhui Agricultural Sciences 2015 (27) 37-38

甘西鼠尾草ISSR-PCR反应体系的建立与优化

作  者:
李文渊;王津慧;童丽;热增才旦;杨芳;索南邓登
单  位:
青海大学医学院药学系
关键词:
甘西鼠尾草;ISSR-PCR;优化
摘  要:
采用单因素试验和4因素3水平的正交试验相结合的方法,建立和优化甘西鼠尾草稳定的ISSR-PCR反应体系。结果表明,甘西鼠尾草20 ul ISSR-PCR体系中各因素的最佳浓度:10×Buffer(Mg2+)2μl,d NTPs 0.4 mmol/L,Tag酶1.0 U,引物0.3μmol/L,DNA 30 ng。
译  名:
Establishment and Optimization of Salvia przewalskii Maxim. ISSR-PCR Reaction System
作  者:
LI Wen-yuan;WANG Jin-hui;TONG Li;Medical College of Qinghai University;
关键词:
Salvia przewalskii Maxim;;ISSR-PCR;;Optimization
摘  要:
Using the single factor test and the 4 factors and 3 level orthogonal test,the ISSR-PCR reaction system and amplification program for Salvia przewalskii Maxim were established. The study showed that the optimum concentration for each factor in 20 μl ISSR-PCR reaction system were 10 × Buffer( Mg2 +) 2 μl,d NTPs 0. 4 mmol/L,Tag enzyme 1. 0 U,primer 0. 3 μmol/L,DNA30 ng.

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