当前位置: 首页 > 文章 > 耐辐射球菌清除活性氧自由基及对DNA的保护作用 核农学报 2004,18 (5) 46-50
Position: Home > Articles > FREE RADICALS SCAVENGING EFFECTS OF Deinococcus radiodurans PREVENTS OXIDATIVE DAMAGE TO DNA Journal of Nuclear Agricultural Sciences 2004,18 (5) 46-50

耐辐射球菌清除活性氧自由基及对DNA的保护作用

作  者:
田兵;徐步进;华跃进
单  位:
浙江大学原子核农业科学研究所
关键词:
耐辐射球菌;活性氧自由基;辐射;DNA损伤;抗氧化酶
摘  要:
用化学发光法分析了耐辐射球菌 (D .radiodurans)的两个菌株 (R1、KD830 1 )和大肠杆菌对超氧阴离子自由基、羟基自由基和过氧化氢的清除能力。通过羟基自由基诱导的DNA氧化损伤化学发光模型考察了耐辐射球菌细胞提取物对自由基氧化导致DNA氧化损伤的保护作用。结果表明 ,耐辐射球菌提取物能显著清除O-2·、H2 O2 和·OH ,清除能力明显高于大肠杆菌 ,并能有效地防止自由基所致DNA氧化损伤 ,表现出超强的抗氧化和耐辐射能力 ,这归因于耐辐射球菌中具有较高的抗氧化酶活性
译  名:
FREE RADICALS SCAVENGING EFFECTS OF Deinococcus radiodurans PREVENTS OXIDATIVE DAMAGE TO DNA
作  者:
TIAN Bing XU Bu jin HUA Yue jin (Institute of Nuclear Agricultural Sciences, Key Lab of Nuclear Agricultural Sciences, Ministry of Agriculture of P.R.China, Zhejiang University, Hangzhou, Zhejiang,\ 310029)
关键词:
Deinococcus radiodurans; oxygen free radicals; irradiation; DNA damage; antioxidant enzyme
摘  要:
Scavenging effects of Deinococcus radiodurans R1,KD8301 and Escherichia coli against O - 2·,H 2O 2 and ·OH were investigated with chemiluminescence method. Hydroxyl radical mediated DNA damage chemiluminiscence system was used to determined whether the extracts from Deinococcus radiodurans had protective effect on free radical induced oxidative damage to DNA.The extract from Deinococcus radiodurans showed higher activities in all experiments than extract from Escherichia coli, suggesting that it had stronger antioxidant activities and radioresistance. It is indicated that the strong scavenging effects on free radical and protective effects on DNA of Deinococcus radiodurans are resulted from its higher activities of antioxidant enzymes.

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