中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 产酶溶杆菌OH11菌株胞外酶调控相关基因ctp的克隆及功能的初步分析 中国生物防治学报 2011,27 (1) 68-75
Position: Home > Articles > Cloning and Funcitional Analysis of Lytic Enzyme Production Related Gene ctp in Lysobacter enzymogenes Strain OH11 Chinese Journal of Biological Control 2011,27 (1) 68-75

产酶溶杆菌OH11菌株胞外酶调控相关基因ctp的克隆及功能的初步分析

作  者：
蒋淑贞;钱国良;刘轶儒;范加勤;胡白石;刘凤权
单  位：
南京农业大学植物保护学院/农业部病虫监测与治理重点开放实验室
关键词：
产酶溶杆菌;胞外酶调控;基因克隆;敲除;功能分析
摘  要：
利用mariner转座子对产酶溶杆菌Lysobacterenzymogenes OH11进行转座诱变,构建菌株OH11的突变体文库。筛选到1株4种胞外酶(蛋白酶、纤维素酶、几丁质酶和β-1,3-葡聚糖酶)产生均减少的突变株D-11。通过亚克隆,鉴定转座子的插入位点,涉及1个羧基末端蛋白酶(carboxy-terminal protease)编码基因ctp。通过同源重组的方法对该基因进行敲除,对缺失突变体Δctp表型分析发现:(1)Δctp与野生型OH11在营养丰富型(2YT)与营养缺陷型(MMX)培养基中生长速率基本一致;(2)Δctp降低了蛋白酶、纤维素酶和β-1,3-葡聚糖酶的产生,但不影响几丁质酶的产生;(3)Δctp生物膜的产生量明显减少。研究还表明,突变体基本不改变其对油菜菌核病菌Sclerotinia sclerotiorum、水稻纹枯病菌Rhizoctonia solani和辣椒疫霉病菌Phytophthora capsici的拮抗能力。互补菌株均恢复了野生型的相关功能。
译  名：
Cloning and Funcitional Analysis of Lytic Enzyme Production Related Gene ctp in Lysobacter enzymogenes Strain OH11
作  者：
JIANG Shu-zhen,QIAN Guo-liang,LIU Yi-ru,FAN Jia-qin,HU Bai-shi,LIU Feng-quan(Key Laboratory of Monitoring and Management of Plant Disease and Insects,Ministry of Agriculture;Department of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China)
关键词：
Lysobacter enzymogenes;regulation of lytic enzyme;gene cloning; knock out;functional analysis
摘  要：
The mutant library of Lysobacter enzymogenes strain OH11 was successfully constructed by mating mariner transposon into strain OH11.One mutant D-11,which reduced the activities of several lytic enzymes,including α-lytic protease,cellulase,chitinase and β-1,3-glucanase,was selected from over 5000 chloramphenicol-resistant(Cmr) mutants.The transposon insertion site was identified as a ctp gene by subclone strategy.Sequence analysis showed that the gene was 2214 bp with a 69% G+C content.The OH11 ctp homology analysis indicated that the homology between OH11 and Stenotrophomonas maltophilia strain was 79%,and that between OH11 and Xanthomonas campetris strain was 78%.A ctp deletion mutant Δctp was constructed by homologue recombination technology.Phenotype analysis revealed that Δctp had the same growth rate as that of wild-type OH11 in both nutrient-rich medium(2YT) and nutrient-deficient medium(MMX).Compared with wild-type OH11,Δctp significantly reduced production of three-type lytic enzymes, i.e.α-lytic protease,cellulase and β-1,3-glucanase,but had the wild-type ability in chitinase production,and Δctp also showed an obvious reduction of biofilm formation.However,Δctp displayed wild-type antimicrobial activity against Sclerotinia sclerotiorum,Rhizoctonia solani and Phytophthora capsici. All phenotypes of mutant were restored in complemented strain.

相似文章

导出引用
计量
文章访问数: 16
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46