中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > β-hemA-ISCOMs对鳗鲡的口服免疫效果 福建农业学报 2006,21 (4) 31-35
Position: Home > Articles > Immunity level of the eel after oral immunized with β-hemA-ISCOMs Fujian Journal of Agricultural Sciences 2006,21 (4) 31-35

β-hemA-ISCOMs对鳗鲡的口服免疫效果

作  者：
龚晖;林天龙;吴宗福;宋铁英
单  位：
福建省农业科学院生物技术研究所
关键词：
嗜水气单胞菌;β-hemA-ISCOMs;口服免疫;淋巴细胞;免疫保护
摘  要：
携带嗜水气单胞菌β-溶血素基因的重组菌可表达具天然活性的β-溶血素。重组菌胞外产物制成的ISCOMs(β-hemA-ISCOMs)经由肠胃途径免疫,可显著提高鳗鲡淋巴细胞的增殖转化能力(P<0.05),并诱导免疫保护,一次免疫的攻毒存活率为44.4%。二次免疫的攻毒存活率为100%。本研究首次将ISCOMs技术应用于鳗鲡口服免疫,试验结果表明ISCOMs技术在发展鱼类口服型疫苗上有广阔的应用前景。
译  名：
Immunity level of the eel after oral immunized with β-hemA-ISCOMs
作  者：
GONG hui~(1,2),LIN Tian-long~(1),WU Zong-fu~(1),SONG Tie-ying~(1)(1.Biotechnology Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China;2.Aquatic Pathogen Collection Center of Ministry of Agriculture,Shanghai Fisheries University,Shanghai 200090,China)
关键词：
Aeromonas hydrophila;β-hemA-ISCOMs;oral immunization;lymphocyte;immunological protection
摘  要：
The recombinant plasmid containing completed β-hemolysin gene of Aeromonas hydrophila was transformed into E.coli DH5α and could express βhemolysin with native biological function.The ISCOMs included crude purified transformant's extracellular products(ECPs) was made and named β-hemA-ISCOMs.The result showed it could enhanced the proliferation ability of eel's lymphocyte and induced immunity significantly by oral administration(P<0.05),after challenge,the livability of first immunized was 44.4%.the livability of second immunized was 100%.This was, to our knowledge,the first report about ISCOMs used in eel's oral administration,indicated that ISCOMs technology could be widely used for producing fish oral vaccines.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46