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当前位置: 首页 > 文章 > H_2O_2诱导BRL-3A细胞模拟大鼠肝脏氧化应激损伤的研究 中国兽医学报 2013,33 (3) 445-449
Position: Home > Articles > Simulating oxidative stress damage of rat liver through H_2O_2 induction on BRL-3A cells Chinese Journal of Veterinary Science 2013,33 (3) 445-449

H_2O_2诱导BRL-3A细胞模拟大鼠肝脏氧化应激损伤的研究

作  者：
臧琳;郭景茹;郭爽;王慧;计红;甄莉;汪志;杨焕民
单  位：
天津嘉立荷牧业有限公司;黑龙江八一农垦大学动物科技学院;吉林大学畜牧兽医学院
关键词：
过氧化氢;BRL-3A细胞;冷暴露;肝脏;氧化应激
摘  要：
动物冷暴露过程中,肝脏因发生氧化应激而受损。利用不同浓度过氧化氢(H2O2)作用BRL-3A细胞,根据细胞存活率确定500μmol/L H2O2作用细胞3h为施加条件,然后通过检测蛋白质羰基含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性,探讨在细胞水平上建立冷暴露大鼠肝脏损伤模型。结果显示,H2O2处理组细胞存活率下降,蛋白质羰基含量升高,SOD、GSH-Px和CAT活性均降低。与对照组相比,其存活率、细胞内蛋白质羰基含量和CAT活性,均差异极显著(P<0.01),细胞内SOD和GSH-Px活性,差异显著(P<0.05)。结果表明,500μmol/L H2O2作用于BRL-3A细胞可导致氧化应激的发生,其相关指标与大鼠冷暴露后肝脏受损指标改变趋势类似,利用BRL-3A细胞建立冷暴露引起的肝脏氧化损伤模型,为进一步探讨冷应激对肝脏损伤的机制奠定基础。
译  名：
Simulating oxidative stress damage of rat liver through H_2O_2 induction on BRL-3A cells
作  者：
ZANG Lin1,GUO Jing-ru2,GUO Shuang1,WANG Hui1,JI Hong1,ZHEN Li1,WANG Zhi3,YANG Huan-min1(1.College of Animal Science &Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China;2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;3.Tianjin Jialihe Livestock Co.Ltd.,Tianjin 300402,China)
关键词：
hydrogen peroxide;BRL-3A cell;cold exposure;liver;oxidative stress
摘  要：
The animal liver can be damaged by oxidative stress during cold exposure.In this study,different doses of hydrogen peroxide(H2O2) were used to treat BRL-3A cells,and the optimal concentration was then chosen by measuring the cell survival rate.In this way,we selected the 500 μmol/L H2O2 as an induced concentration acting on BRL-3A cells for 3 h,then tried to establish an experimental BRL-3A cell model of liver damages in rat simulating cold exposure using H2O2,to detect the protein carbonyl content,and the activities of superoxidase dismutase(SOD),glutathione peroxidase(GSH-Px) and catalse(CAT).Results showed that in the H2O2-treated group,a decrease in cell survival rate,an increase in protein carbonyl content and a decrease in SOD,GSH-Px and CAT activities were observed.And comparing to the control group,a highly significant difference(P<0.01) was found in cell survival rate,protein carbonyl content and CAT activity,and a rather significant difference(P<0.05) in SOD and GSH-Px activities.Thus,the results proved that an oxidative stress could be generated when H2O2 actied on BRL-3A cells,and the change trends of indicators were similar to those of a real cold-exposed-and-liver damage in rat.Thereby,this study makes it possible to establish a cell model of stimulating hepatic oxidative damage by cold exposure using H2O2 induction on BRL-3A cells,which will set up a solid base for the future studies on the other relative mechanisms on cold stress.

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