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当前位置: 首页 > 文章 > 家蚕性附腺发育与核酸及利它素蛋白变化的关系 昆虫学报 2002,45 (5) 25-29
Position: Home > Articles > The relationships between development, nucleic acids and kairomone proteins of Bombyx mori female accessory glands Acta Entomologica Sinica 2002,45 (5) 25-29

家蚕性附腺发育与核酸及利它素蛋白变化的关系

作  者：
高其康;楼兵干;董海涛;胡萃
单  位：
浙江大学应用昆虫研究所;浙江大学生物技术研究所;浙江大学分析测试中心;浙江大学植物保护系
关键词：
家蚕;野蚕黑卵蜂;利它素;核酸
摘  要：
对不同发育时期家蚕Bombyxmori雌性性附腺核酸和蛋白质含量测定的结果表明 ,从家蚕化蛹后第 6天到成虫羽化当天的性附腺内总蛋白质含量不断增加 ,至羽化当天为最高 ,达 86 0± 70 μg 对。不同时期内能引诱野蚕黑卵蜂识别寄主的利它素在总蛋白中所占的比例差异明显 ,从化蛹后第 6天的 10 %增加到羽化后的 5 8%。性附腺的总RNA含量从化蛹第 6天到成虫羽化前 1天几乎是直线增加 ,但羽化后下降很快。不同时期分泌部中总RNA含量变化与贮存部明显不同。含量最高时 ,分泌部RNA可占整个性附腺RNA的 90 %以上 ,而贮存部总RNA含量则远少于分泌部 ,羽化当天约为分泌部的十分之一。分泌部总RNA中的 18S亚基含量远高于 2 8S亚基 ,明显不同于贮存部的。从分泌部总RNA中分离的mRNA存在明显的条带分布 ,这预示着高丰度的mRNA的存在与总蛋白中高含量利它素的存在有着特殊对应关系。
译  名：
The relationships between development, nucleic acids and kairomone proteins of Bombyx mori female accessory glands
作  者：
GAO Qi\|Kang 1,2 , LOU Bing Gan 3, DONG Hai Tao 4, HU Cui 2 (1 Center of Analysis and Measurement, Zhejiang University, Hangzhou 310029, China; 2 Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China; 3 Department
关键词：
Bombyx mori;Telenomous theophilae; host recognition kairomone; nucleic acids
摘  要：
Based on changes in nucleic acids and proteins during the development of Bombyx mori female accessory glands, it was determined that the protein content in these glands increased gradually from the sixth day after pupation to day of eclosion, peaking on the day of eclosion (860±70 μg/pair). The ratio of host Telenomus theophilae recognition kairomone to total protein varied significantly at different development stages, from 10% on the sixth day after pupation to 58% on the day of eclosion. The amount of total RNA increased from the sixth day after pupation to the day just before eclosion, but decreased quickly after eclosion. The quantity of RNA varied significantly between the secretory and reservoir portions. In the secretory portion, the amount of total RNA changed significantly during the development of the accessory glands. At its peak, the total RNA in the secretory portion accounted for more than 90% of the total RNA in the accessory glands. In contrast, the amount of total RNA in the reservoir portion was much less than that in the secretory portion and did not change significantly through development. The total RNA in the reservoir portion was only 1/10 as much as in the secretory portion on the day before eclosion. Agarose gel electrophoresis indicated that the quantity of 18S rRNA was much higher than that of 28S rRNA in the secretory portion. Certain sizes of extracted mRNA were relatively abundant, suggesting that these kinds of mRNA are specifically related to certain major secretory proteins.

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