中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 紫丁香天然群体遗传多样性的AFLP分析 园艺学报 2006,33 (6) 1269-1274
Position: Home > Articles > Genetic Diversity in Natural Populations of Syringa oblata Detected by AFLP Markers Acta Horticulturae Sinica 2006,33 (6) 1269-1274

紫丁香天然群体遗传多样性的AFLP分析

作  者：
明军;顾万春
单  位：
中国农业科学院蔬菜花卉研究所;中国林业科学研究院林业研究所
关键词：
紫丁香;天然群体;AFLP;遗传多样性
摘  要：
抽取内蒙古大黑山、辽宁北票和山西五老峰3个紫丁香(Syringa oblataLindl.)天然群体为拟似群体,用筛选出的8对引物,对群体家系组成的72个样品进行AFLP分析。Nei(1973)基因多样性指数h=0.2287,Shannon s信息指数I=0.3464;种级遗传多样性Ht=0.3522,群体内遗传多样性Hs=0.2287,群体间遗传多样性Dst=0.1235,群体分化系数Gst=0.3508,群体间总的基因流的估算值Nm=0.9253,群体遗传变异的AMOVA分析表明群体的遗传多样性主要分布在群体间,群体间方差分量的贡献率占51.53%。基于Nei(1972)遗传距离的UPGMA聚类分析显示群体间的遗传距离与群体的地理距离关系一致。结果表明:紫丁香群体间的遗传分化较大,其天然群体缺失造成的现有分布的间断性和地理隔离以及高自交率是导致其群体间的高度分化的主要因素。在目前分布残缺,群体丢失严重的情况下,紫丁香种质资源保护和利用策略应为采取优先保存尽可能多的群体。
译  名：
Genetic Diversity in Natural Populations of Syringa oblata Detected by AFLP Markers
作  者：
Ming Jun 1* and Gu Wanchun 2(1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China)
关键词：
Syringa oblata;Natural population;Amplified Fragment Length Polymorphism(AFLP);Genetic diversity
摘  要：
Seeds were collected from 30 trees selected from each of the 3 natural populations of Syringa oblata Lindl.located respectively in Daheishan mountain in Inner Mongolia Autonomous Region,Beipiao in Liaoning Province and Wulaofeng in Shanxi Province.72 wild accessions of the collections were used in this study.Seeds were germinated and a bulk sample of 20 leaves was collected from each family for molecular marker analysis.Eight EcoRⅠ-MseⅠ AFLP primer combinations revealed 864 legible bands of which 187 were polymorphic markers.Nei's genetic diversity was 0.2287,Shannon's information index was 0.3464,genetic diversity at species level was 0.3522,observed heterozygosity was 0.2287 within population and 0.1235 among populations,coefficient of genetic differentiation was 0.3508 and the average gene flow estimated from Gst was 0.9253.The results of AMOVA indicated that the variance component of among populations contributes 51.53% to the total genetic variance.UPGMA cluster analysis based on Nei's genetic disitance indicated that genetic distances were closely related to corresponding geographic distances among populations.The results also indicated that genetic differentiation among populations was large,mainly attributable to geographic isolation due to the discontinuous distribution of the species and high rate of selfing.Conservation strategy for the species should be therefore emphasized on conserving as many as possible the existing populations considering the current conditions of severe population fragmentation and losses.

相似文章

导出引用
计量
文章访问数: 18
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46