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当前位置: 首页 > 文章 > 混合感染后杆状病毒间的增效作用──病毒蛋白及核酸的初步分析 昆虫学报 1997,40 (1) 7-14
Position: Home > Articles > THE SYNERGISTIC ACTION BETWEEN BACULOVIRUSES AFTER MIXED INFECTION──A PRIMARY ANALYSIS OF VIRAL PROTEINS AND NUCLEIC ACIDS Acta Entomologica Sinica 1997,40 (1) 7-14

混合感染后杆状病毒间的增效作用──病毒蛋白及核酸的初步分析

作  者：
王燕;丁翠;蔡秀玉
单  位：
中国科学院动物研究所
关键词：
核型多角体病毒;黄地老虎;烟青虫;棉铃虫;粘虫;混合侵染;增效
摘  要：
AsNPV＋HasNPV、AsNPV＋HaNPV、AsNPV＋PsNPV分别感染烟青虫、棉铃虫和粘虫幼虫，对分离到的核型多角体病毒（AsNPV＋HasNPV）－Helicoverpaassulta、（AsNPV＋HaNPV）－H．armigera和（AsNPV＋PsNPV）－Pseudaletiaseparata，经电镜观察，多角体蛋白及病毒粒子蛋白SDS－PAGE电泳，病毒核酸的限制性内切酶酶解分析等研究，证明各病毒的多角体形态不规则，大小差异极大，病毒粒子为杆状，（AsNPV＋HasNPV）－H．assulta和（AsNPV＋HaNPV）－H．armigera病毒粒子有单粒和多拉包埋类型，（AsNPV＋PsNPV）－P．separata为多粒包理型。各病毒的多角体蛋白基本上只有一种多肽，分子量为25000道尔顿左右。（AsNPV＋HasNPV）－H.assulta、（AsNPV＋HaNPV）－H.armigera和（AsNPV＋PsNPV）－P．separata的病毒粒子分别有10、14、5条多肽，分子量大小在13500～98000，13000～88000，18500～52000道尔顿之间。病毒核酸经EcORI，HindIII，HindIII＋BamHI酶解，其DNA的酶切位点，大小及DNA的总分子量与AsNPV和原寄主的Has－NPV，HaNPV和PsNPVDNA的酶切图谱存在一定差异，混合病毒侵染昆虫后新复制的病毒核酸发生一定的变化，从而导致病毒蛋白和病毒形态的变化。混合感染后AsNPV对Has－NPV、HaNPV和PsNPV的侵染有明显?
译  名：
THE SYNERGISTIC ACTION BETWEEN BACULOVIRUSES AFTER MIXED INFECTION──A PRIMARY ANALYSIS OF VIRAL PROTEINS AND NUCLEIC ACIDS
作  者：
Wang Yan; Ding Cui; Cai Xiuyu (Institute of Znology, Academia Sinica Beijing 100080)
关键词：
nuclear polyhedrosis virus (NPV), Agrotis segetum,Helicoverpa assulta, Helicoverpa armigera, Pseudaletia separata, mixed infection, enhancement
摘  要：
AsNPV was mixed with HasNPV, HaNPV and PsNPV separately and the three mixtures were used to infect the larvae of Helicoverpa assulta, H. armiera and Pseu- daletia separata respectively. The nuclear polyhedrosis viruses thus obtained were called (AsNPV + HasNPV) -H. assulta, (AsNPV +HaNPV) -H. armlerra and (AsNPV + PsNPV ) -P. separata respectively. The polyhedra of the NPVs were in irregular shape and varied distinctly in sizes. The biochemical charcaters of the viruses were investigated and compared with those of HasNPV, HaNPV and PsNPV. The polyhedrin and viral structural polypeptides of the NPVs were examined by using SDS-PAGE. The polyhedrin of all NPVs had molecular weight of about 25 000 dalton. The numbers of the viral structural polypep- tides of (AsNPV+HasNPV)-H. assulta, (AsNPV+HaNPV)-H. armigera and (AsNPV + PsNPV) -P. separata were 10, 14, 5 respectively, and their molecular weights were 13 500~ 98 000, 13 000~ 88 000, 18 500~ 52 000 dalton. The analysis of agarose elec- trophoretic profiles of DNAs restriction endonuclease fragments showed that they were not identical to those of AsNPV, HasNPV, HaNPV and PsNPV. The DNAs of the viruses had changed and resulted in the changes of viral proteins and viral morphogenesis. Agrotis sege- tum nuclear polyhedrosis virus (AsNPV) can enhance the infection of HasNPV, HaNPV and PsNPV. The enhancement mechanism of AsNPV is worthwhile to be further investi- gated.

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