当前位置: 首页 > 文章 > 史密斯桉组织培养技术及褐变问题研究 湖南农业大学学报(自然科学版) 2008,34 (1) 68-71
Position: Home > Articles > Researches on tissue culture and browning prevention of Eucalyptus smithii Journal of Hunan Agricultural University(Natural Sciences) 2008,34 (1) 68-71

史密斯桉组织培养技术及褐变问题研究

作  者:
饶红欣;蒋利媛;彭信海;夏晓敏;刘先芳
单  位:
湖南省森林植物园
关键词:
史密斯桉;组织培养;褐变
摘  要:
对史密斯桉(Eucalyptus smithii)的组织培养和褐变控制等进行探讨,结果表明:初接种采用MS+BA 0.2 mg/L+IBA 1 mg/L培养基,芽诱导率为40%;继代采用MS+BA 0.2 mg/L+IBA 0.8 mg/L培养基,增殖系数可达4,芽抽茎成苗率30.1%;生根采用MS+IBA 2 mg/L培养基,根诱导率36.7%,平均生根2.4条.培养基中BA质量浓度为0.2~1.0 mg/L,有利于芽的生长,但超过1.0 mg/L时反而抑制芽的生长.不同培养基添加物减轻褐变的效果不同,而同种添加物不同浓度的效果也不一样,加入50 mg/L PVP或100 mg/L V-C能显著减轻褐变,褐变率分别降低10%和8.3%.暗培养法虽能有效控制褐变,但严重影响愈伤组织和芽的生长,不宜采用.
译  名:
Researches on tissue culture and browning prevention of Eucalyptus smithii
作  者:
RAO Hong-xin,JIANG Li-yuan,PENG Xin-hai,XIA Xiao-min,LIU Xian-fang(Hunan Provincial Forest Botanic Garden,Changsha 410116,China)
关键词:
Eucalyptus smithii;tissue culture;browning
摘  要:
Researches on tissue culture and browning prevention of Eucalyptus smithii were conducted,and the results showed:initial shoot induction rate for medium,MS+BA 0.2 mg/L+IBA 1 mg/L,was 40% during initial inoculation;shoot multiplication rate and shoot formation rate for medium,MS+BA 0.2 mg/L+IBA 0.8 mg/L,were 4 and 30.1% respectively during subpassage;root induction rate and mean rooting number for medium,MS+IBA 2 mg/L,was 36.7% and 2.4 respectively during rooting period.BA supplemented with concentration 0.2~1.0 mg/L promoted shooting,but restrained it when higher than 1.0 mg/L on the other hand.Different supplements had different effects in lightening bowing,so was the same kind of but differently-concentrated supplements.50 mg/L PVP or 100 mg/L V-C could lighten browning significantly by 10% and 8.3% respectively.It’s better not to adopt all-dark cultivation because it restrained the growth of callus and shoots badly,in spite that it could prevent browning effectively.

相似文章

计量
文章访问数: 5
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊