Position: Home > Articles > Primary Study on Bacteriostatic Active Compound from Leaves of Loropetalum chinense var. rubrum Yieh
FOOD SCIENCE
2007,28
(6)
74-77
红檵木叶中抑菌活性成分的初步研究
作 者：
周国海;于华忠;卢成英;周哲
单 位：
吉首大学城乡资源与规划学院;湖南省林产化工工程重点实验室
关键词：
红檵木叶;抑菌;活性成分;薄层层析
摘 要：
本研究以红檵木叶为材料,对其抑菌活性成分进行提取,将提取液用不同溶剂分步萃取;以金黄色葡萄球菌、大肠杆菌和痢疾杆菌为供试菌种,进行抑菌实验;对抑菌活性较强的萃取液进行薄层层析分离,摸索最佳展开剂,并对薄层层析分离所得抑菌活性成分进行抑菌实验和化学检识,以研究红檵木叶中抑菌活性成分的种类。结果表明:乙酸乙酯为最佳萃取溶剂;氯仿:丙酮:甲酸:水(60:35:5:2)为最佳展开剂;红檵木叶中所含抑菌活性成分主要为鞣质类和香豆素类。
译 名：
Primary Study on Bacteriostatic Active Compound from Leaves of Loropetalum chinense var. rubrum Yieh
作 者：
ZHOU Guo-hai1,YU Hua-zhong2,LU Cheng-ying2,ZHOU Zhe1 (1.College of Resources and Planning Sciences, Jishou University, Zhangjiajie 427000, China; 2.Key Laboratory of Hunan Forest Products Chemical Engineering, Zhangjiajie 427000, China)
关键词：
leaves of Loropetalum chinense var. rubrum Yieh;bacteriostasis;active compounds;thin layer chromatography
摘 要：
In this paper, the leaves of Loropetalum chinense var. rubrum Yieh were used as raw material, and the bacteriostatic active compound with different solvents extracted in turn. The bacteriostatic effects on Staphylococlus aureus, Shigella dysenteroae and Escheichia colis were studied with bacteriostatic experiment. The bacteriostatic active extract was separated by thin layer chromatography. The best developer is found. Then the active compounds separated by thin layer chromatography were tested by the bacteriostatic experiment and chemical recognition so as to affirm the species of bacteriostasis active compounds. The results showed that ethyl acetate is the best solvent and the best developer solvent is: chloroform: acetone: formic acid: water (60:35:5:27 respectively). The main active compounds in the leaves of Loropetalum chinense var. rubrum Yieh are tannin and coumarin.
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