Position: Home > Articles > Study on Extract and Separate Bacteriostatic Active Composition from Loropetalum Chinese var. rubrum Leaves
FOOD SCIENCE
2007,28
(10)
225-228
红檵木叶抑菌活性物提取分离研究
作 者：
钟以举;唐克华;钟维;黄早成;卢成瑛
单 位：
吉首大学湖南省林产化工工程重点实验室
关键词：
红檵木叶;抑菌活性;柱层析;薄层层析;可水解鞣质
摘 要：
用不同浓度乙醇作提取溶剂,采用正交试验,通过抑菌实验得到红檵木叶抑菌活性物质最佳提取条件为:60%乙醇、45℃、3h。提取物对金黄色葡萄球菌的最小抑菌浓度为12.5mg/ml,对痢疾杆菌为50mg/ml,对大肠杆菌为25mg/ml。提取液抑菌活性具热稳定性,但在碱性条件下不稳定。溶剂萃取以乙酸乙酯萃取物抑菌活性最强,硅胶柱层析和薄层层析进一步分离,得到三个抑菌活性较强的组分,化学反应和UV检测表明其属可水解鞣质类。
译 名：
Study on Extract and Separate Bacteriostatic Active Composition from Loropetalum Chinese var. rubrum Leaves
作 者：
ZHONG Yi-ju,TANG Ke-hua,ZHONG Wei,HUANG Zao-cheng,LU Cheng-ying (Provincial Key Laboratory of Forest Production and Chemical Engineering, Jishou University, Zhangjiajie 427000, China)
关键词：
Loropetalum Chinese var. rubrum leaves;bacteriostatic activity;column chromatography;TLC; hydrolysablet annin
摘 要：
Antibioticsubstance of Loropetalum Chinese var. rubrum leaves were extracted by the ethanol in orthogonal test. The optimum extracting conditions are: 60% ethanol , 45℃ and 3h. Extract acted on Staphylococlus aureus, Shigella dysenteroae and Escheichia coli, the MIC was 12.5 mg/ml, 50 mg/ml and 25 mg/ml respectively. Extracting solution was not steady in the alkaline environment, but was steady in the hi-temp. Antibioticsubstance could be extracted by the ethyl acetatehe three bacteriostatic active composition were isolated with silica gel chromatography and thin-layer chromatography (TLC) in the extract of ethyl acetate. The result of chemical reaction and the examination of UV showed that extracts are hydrolysablet annin.
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