中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 蒙古黄芪的组织培养及辐射诱变研究 安徽农业科学 2009,37 (9) 3939-3941
Position: Home > Articles > Study on Tissue Culture and Radiation Mutation of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao Journal of Anhui Agricultural Sciences 2009,37 (9) 3939-3941

蒙古黄芪的组织培养及辐射诱变研究

作  者：
赵秀娟;张红霞
单  位：
内蒙古科技大学生物与化学工程学院
关键词：
蒙古黄芪;组织培养;辐射诱变
摘  要：
[目的]研究蒙古黄芪的组织培养技术及辐射诱变对愈伤组织诱导的影响。[方法]以蒙古黄芪无菌苗的不同外植体(叶片、子叶、下胚轴)进行愈伤组织诱导,并对子叶和下胚轴进行辐射诱变研究。[结果]3种外植体分别在MS+6-BA 2.0 mg/L+NAA 2.0 mg/L、LS+6-BA 2.0 mg/L+NAA 0.1 mg/L、MS+6-BA 2.0 mg/L+NAA 2.0 mg/L培养基上的愈伤组织诱导率较高;下胚轴和子叶的最佳诱变时间均为15 min;随辐射诱变时间的增加,下胚轴愈伤组织的长势增强,但超过一定的诱变时间愈伤组织长势减弱且诱导率降低。[结论]该研究为蒙古黄芪的组织培养和诱变育种提供科学依据。
译  名：
Study on Tissue Culture and Radiation Mutation of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao
作  者：
ZHAO Xiu-juan et al (Biological and Chemical Engineering School,Inner Mongolia University of Science and Technology,Baotou,Inner Mongolia 014010)
关键词：
Astragalus membranaceus Bge.var.mongholicus(Bge.) Hsiao;Tissue culture;Radiation mutation
摘  要：
[Objective] The research aimed to study the tissue culture technology and callus induction by radiation mutation of Astragalus membranaceus Bge.var.mongholicus(Bge.) Hsiao.[Method] With the different parts of Astragalus membranaceus Bge.var.mongholicus(Bge.) Hsiao aseptic seedling as explants(leaves,cotyledons,hypocotyls) induced callus,and cotyledon and hypocotyls taken by the method of radiation mutation were studied.[Result]The results showed that the three explants had relatively high callus induced rate in the medium which respectively made up of MS+6-BA 2.0 mg/L+NAA 2.0 mg/L,LS+6-BA 2.0 mg/L+NAA 0.1 mg/L,MS+6-BA 2.0 mg/L+NAA 2.0 mg/L;the best mutation time of hypocotyls and cotyledons was 15 minutes;the growth of the callus induced from hypocotyls would be better as the mutation time increased,but when it reached a certain time the growth would be weaken,the induction rate also would be reduced.[Conclusion]This study will provide the scientific reference in tissue culture and mutation breeding of Astragalus membranaceus Bge.var.mongholicus(Bge.) Hsiao.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46