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Position: Home > Articles > Comparative Analysis of High-salt Methods of Genomic DNA Extraction from Astragalus membranaceus Hubei Agricultural Sciences 2011,50 (3) 173-176

高盐方法提取蒙古黄芪基因组DNA比较分析

作  者:
韩雅楠;赵秀娟;李艳君;蔡禄
单  位:
内蒙古科技大学数理与生物工程学院
关键词:
高盐;蒙古黄芪;DNA提取
摘  要:
为了提取高质量的蒙古黄芪(Astragalus membranaceus)基因组DNA,采用高盐十二烷基硫酸钠(SDS)法、高盐溴化十六烷基三甲基溴化铵(CTAB)法和高盐低pH值法,结果表明高盐CTAB法获得的黄芪基因组DNA纯度高,能用于PCR扩增和限制性内切酶酶切,是提取黄芪基因组总DNA的最佳方法。
译  名:
Comparative Analysis of High-salt Methods of Genomic DNA Extraction from Astragalus membranaceus
作  者:
HAN Ya-nan,ZHAO Xiu-juan,LI Yan-jun,CAI Lu(School of Mathematics Physics and Biological Engineering,Inner Mongolia University of Science and Technology,Baotou 014010,China)
关键词:
high-salt;Astragalus membranaceus;DNA extraction
摘  要:
High-salt SDS,high-salt CTAB,and high-salt low pH value methods were used to extract high quality Astragalus membranaceus genomic DNA.The results showed that the genomic DNA with the highest quality was extracted by high-salt CTAB method,and was preferable enough to be used as PCR template and digested by restriction enzyme completely;thus high-salt CTAB was the best DNA extraction method.

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