当前位置: 首页 > 文章 > 发根农杆菌rolC基因的克隆及序列分析 西北林学院学报 2007,22 (2) 72-75
Position: Home > Articles > Cloning of rolC Gene of Ri Plasmid and Sequence Analysis Journal of Northwest Forestry University 2007,22 (2) 72-75

发根农杆菌rolC基因的克隆及序列分析

作  者:
刘兴菊;梁海永;杨敏生;周怀军
单  位:
河北农业大学林学院
关键词:
Ri质粒;rolC基因;克隆
摘  要:
以发根农杆菌30148为材料,设计了rolC基因的特异性引物,应用PCR技术,扩增出了正确序列的rolC基因585 bp片段,并利用pUCm-T载体对此片段进行了克隆,并进行了序列分析。所克隆rolC基因与已发表的序列相比较,核苷酸序列的同源性达到100%,但另一个克隆中部分碱基发生了改变,同源性达到99%。
译  名:
Cloning of rolC Gene of Ri Plasmid and Sequence Analysis
作  者:
LIU Xing-ju,LIANG Hai-yong,YANG Min-sheng*, ZHOU Huai-jun(College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071000,China)
关键词:
Ri plasmid;rolC gene;sequence analysis
摘  要:
Ri plasmid was developed and a 585bp fragment of rolC gene was amplified by PCR via primer of rolC gene,and the fragment was cloned into vector pUCm-T.Comparison with previously published nucleotide sequence showed that the homology was 100%.But another clone gene nucleotide sequence showed that the homology was 99% with it.

相似文章

计量
文章访问数: 33
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊